bs-70024R

DATASHEET

Host: Rabbit

Target Protein: ATRIP (Ser239)

Specificity: Specific for endogenous levels of the ~86 kDa ATRIP protein phosphorylated at Ser239. The antibody also recognizes a band at ~32 kDa.

Modification Site: Ser239

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84126

Swiss Prot: Q8WXE1

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at Ser-239 (Venere et al, 2007).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 86

Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Bovine
Sheep
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.