bs-70020R

DATASHEET

Host: Rabbit

Target Protein: Aquaporin 4

Specificity: Specific for endogenous levels of the ~35 kDa aquaporin 4 protein.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25293

Swiss Prot: P47863

Source: Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat AQP4, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Aquaporin-4 (AQP4), a bidirectional water channel protein, is the most expressed aquaporin within the central nervous system. AQP4 is predominantly expressed by astrocytes and ependymal cells within the blood-brain-barrier and ependymal-cerebrospinal fluid barriers (Verkman, et al 2011). AQP4 plays a role in synaptic plasticity (Skucas et al, 2011), astrocyte mitigation (Saadoun et al, 2005), and K+ homeostasis (Binder et al, 2006). Due to the significant role AQP4 plays in cognition, it has been reported to be dysregulated in several neurological disorders. Alzheimer’s patients have amyloid deposits in the walls of the vasculature known as CAA which causes AQP4 mis-location (Wilcock et al, 2009). Patients with Parkinson’s disease have low levels of AQP4 expression which leads to reduced inflammatory response (Chi et al,2011). Reduced levels of AQP4 in traumatic brain injury affects both the acute stage, decreasing the ability to remove excess water from the brain, and in the later stage, by preventing cellular damage and swelling (Zhang et al, 2015).