bs-70004R

DATASHEET

Host: Rabbit

Target Protein: AIF1/Iba1

Specificity: Specific for endogenous levels of the ~17 kDa AIF1/Iba1 protein.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 199

Swiss Prot: P55008

Source: Full-length human AIF1/Iba1 protein expressed in and purified from E. coli.

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998). During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009). Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 17

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.