bs-70000R

DATASHEET

Host: Rabbit

Target Protein: 14-3-3 Protein

Specificity: Specific for endogenous levels of the ~29 kDa 14-3-3 protein. Immunolabeling is blocked by preadsorption of the antibody with the peptide immunogen.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 56011

Swiss Prot: P35213

Source: Peptide corresponding to amino acid residues from the N-terminal region of the rat 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 29

Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.