bs-6976R [Primary Antibody]
Phospholipase C beta 1 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Phospholipase C beta 1

Immunogen Range: 441-540/1216


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23236

Source: KLH conjugated synthetic peptide derived from human Phospholipase C beta 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phospholipase C beta 1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. Its gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for its gene.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 134


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li Yin. et al. Macrophage P2Y6R activation aggravates psoriatic inflammation through IL-27-mediated Th1 responses. ACTA PHARM SIN B. 2024 JuRead more>>
  • Huifang Niu. et al. Molecular Mechanism of Pasteurized Akkermansia muciniphila in Alleviating Type 2 Diabetes Symptoms. J AGR FOOD CHEM. 2024;72(23):1308313098Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti PLC β1/Phospholipase C beta 1 Polyclonal Antibody, Unconjugated (bs-6976R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Hela cells probed with Phospholipase C beta 1 Antibody, ALEXA FLUOR® 647 Conjugated(bs-6976R-A647) at 1:100 for 30 minutes compared to control cells (blue)and isotype control (orange).


U2OS cell lysates probed with Phospholipase C beta 1 Polyclonal Antibody, Unconjugated (bs-6976R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.