bs-6959R [Primary Antibody]
Estrogen Receptor beta (Ser87) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Estrogen Receptor beta Ser87

Modification Site: Ser87

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2100

Source: KLH conjugated synthetic phosphopeptide derived from human Estrogen Receptor beta around the phosphorylation site of Ser87 [HL(p-S)PL]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER alpha and ER beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ER alpha and ER beta A have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and ER beta may be regulated by distinct mechanisms even though they share many functional characteristics.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 66


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Sheep
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse testis lysates probed with phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody, Unconjugated (bs-6959R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Testis cell lysates; Lane 2: Mouse Ovary cell lysates; Lane 3: Mouse Uterus cell lysates probed with Estrogen Receptor beta (Ser87) Polyclonal Antibody, Unconjugated (bs-6959R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.