bs-6951R [Primary Antibody]
AKT1+2+3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AKT1+2+3

Immunogen Range: 401-480/480


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human AKT1/2/3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu et al. Danshen attenuates cartilage injuries in osteoarthritis in vivo and in vitro by activating JAK2/STAT3 and AKT pathways. (2018) Exp.Anim. 67:127-137Read more>>
  • Jeon et al. Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors. (2018) Sci.Rep. 8:5415Read more>>
  • Han B et al. 8-Cetylcoptisine, a new coptisine derivative, induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest in human A549 cells.(2018)Chem Biol Interact.;299:27-36.Read more>>
  • Liu et al. PD-1/PD-L1 interaction up-regulates MDR1/P-gp expression in breast cancer cells via PI3K/AKT and MAPK/ERK pathways. (2017) Oncotarget. 8:99901-99912Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Wang Y et al. Aspirin inhibits adipogenesis of tendon stem cells and lipids accumulation in rat injury tendon through regulating PTEN/PI3K/AKT signalling. J Cell Mol Med. 2019 Nov;23(11):7535-7544.Read more>>
  • Wang Z et al. Light-activatable dual prodrug polymer nanoparticle for precise synergistic chemotherapy guidedby drug-mediated computed tomography imaging. Acta Biomater. 2019 May 22. Read more>>
  • Xue W et al. Caveolin-1 alleviates lipid accumulation in NAFLD associated with promoting autophagy by inhibiting the Akt/mTOR pathway. Eur J Pharmacol. 2020 Mar 15;871:172910. Read more>>
  • Gao HX et al. TCL1 as a hub protein associated with the PI3K/AKT signaling pathway in diffuse large B-cell lymphoma based on proteomics methods. Pathol Res Pract. 2020 Feb;216(2):152799. Read more>>
  • Yin H et al. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins (Basel). 2020 Jan 29;12(2). Read more>>
  • Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
  • Chen R et al. Tongmai Yangxin pill reduces myocardial no-reflow by regulating apoptosis and activating PI3K/Akt/eNOS pathway. J Ethnopharmacol. 2020 Oct 28;261:113069.Read more>>
  • Di\Yang Sun. et al. iTRAQ\ and LCCMS/MS\based quantitative proteomics reveals Pqlc2 as a potential regulator of hepatic glucose metabolism and insulin signalling pathway during fasting. Clin Exp Pharmacol P. 2021 Feb;48(2):238-249Read more>>
  • Myung-Ju Lee. et al. CXCL1 confers a survival advantage in Kaposi's sarcoma-associated herpesvirus-infected human endothelial cells through STAT3 phosphorylation. J MED VIROL. 2022 JulRead more>>
  • Xusheng Du. et al. RIOK3-mediated Akt phosphorylation facilitates synergistic replication of Mareks disease and reticuloendotheliosis viruses. VIRULENCE. 2022 Jul 06Read more>>
  • Xing Chang. et al. 1-(4-((5-chloro-4-((2-(isopropylsulfonyl)phenyl)amino)pyrimidin-2-yl)amino)-3-methoxyphenyl)-3-(2-(dimethylamino)ethyl)imidazolidin-2-one (ZX-42) inhibits cell proliferation and induces apoptosis via inhibiting ALK and its downstream pathways in Karpas299 cells. TOXICOL APPL PHARM. 2022 Sep;450:116156Read more>>
  • Chuanju Zong. et al. Chronic restraint stress promotes gastric epithelial malignant transformation by activating the Akt/p53 signaling pathway via ADRB2. ONCOL LETT. 2022 Sep;24(3):1-11Read more>>
  • Dongfang Lv. et al. Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.. FRONT PHARMACOL. 2022 Sep;13:961011-961011Read more>>
  • Wen Jiang. et al. Hirsutine ameliorates myocardial ischemia-reperfusion injury through improving mitochondrial function via CaMKII pathway. CLIN EXP HYPERTENS. 2023;45(1):Article: 2192444Read more>>
  • Xiao Feng. et al. Bisbenzylisoquinoline alkaloids from Plumula Nelumbinis inhibit vascular smooth muscle cells migration and proliferation by regulating the ORAI2/Akt pathway. PHYTOCHEMISTRY. 2023 Jul;211:1137Read more>>
  • Ranran Sun. et al. Azadirachtin exposure inhibit ovary development of Spodoptera litura (Lepidoptera: Noctuidae) by altering lipids metabolism event and inhibiting insulin signaling pathways. ECOTOX ENVIRON SAFE. 2023 Sep;262:115151Read more>>
  • Yan Zeng. et al. Effects and mechanism of Rictor interference in podocyte injury induced by high glucose. EXP THER MED. 2023 Oct;26(4):1-9Read more>>
  • Linfeng Zheng. et al. Chitosan Inhibits Vascular Intimal Hyperplasia via LINC01615/MIR-185-5p/PIK3R2 Signaling Pathway. GENE. 2023 Sep;:14785Read more>>
  • Xiaoxiao Zhang. et al. Tanshinone A participates in the treatment of endometriosis by regulating adhesion, invasion, angiogenesis and inhibition of PI3K/Akt/mTOR signaling pathway. MOL MED REP. 2023 Nov;28(5):1-13Read more>>
  • Huijuan Qu. et al. Components study on gastroprotective effect and holistic mechanism of the herbal pair Alpinia officinarum - Cyperus rotundus based on spectrum-effect relationship and integrated transcriptome and metabolome analyses. J ETHNOPHARMACOL. 2023 Nov;:117494Read more>>
  • Yang Yuejie. et al. The effect of bta-miR-1296 on the proliferation and extracellular matrix synthesis of bovine mammary fibroblasts. IN VITRO CELL DEV-AN. 2024 Feb;:1-12Read more>>
  • Amei Tang. et al. Analyzing mechanisms of Qing Fei Bao Yuan Decoction granules in treating COPD based on LC-MS, network pharmacology and in vivo methods. Journal of Traditional and Complementary Medicine. 2024 ApRead more>>
  • Yu Xiong. et al. Positive effect of peptide-calcium chelates from Grifola frondosa on a mouse model of senile osteoporosis. J FOOD SCI. 2024 ApRead more>>
  • Yaji Li. et al. CircMYBL1 suppressed acquired resistance to osimertinib in non-small-cell lung cancer. CANCER GENET-NY. 2024 Jun;284-285:34Read more>>
  • Wang Yunyun. et al. Iridoids rich fraction from Valeriana jatamansi Jones promotes axonal regeneration and motor functional recovery after spinal cord injury through activation of the PI3K/Akt signaling pathway. FRONT MOL NEUROSCI. 2024 May;17Read more>>
  • Fu Yuhang. et al. Effect of umbilical cord blood-mononuclear cells on knee osteoarthritis in rabbits. J ORTHOP SURG RES. 2024 Dec;19(1):1-9Read more>>
VALIDATION IMAGES

HepG2 lysate probed with Rabbit Anti-AKT1+2+3 Polyclonal Antibody (bs-6951R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Mouse heart lysates probed with AKT1+2+3 Polyclonal Antibody, Unconjugated (bs-6951R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Liver lysates; Lane 2: Mouse Lung lysates probed with AKT1+2+3 Polyclonal Antibody, Unconjugated (bs-6951R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT1+2+3) Polyclonal Antibody, Unconjugated (bs-6951R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.