Host: Rabbit
Target Protein: Cyclin M2
Immunogen Range: 51-150/875
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 54805
Swiss Prot: Q9H8M5
Source: KLH conjugated synthetic peptide derived from human Cyclin M2/CNNM2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 96
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Takashina Y et al. Sodium Citrate Increases Expression and Flux of Mg Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells.(2018) Nutrients. Sep 20 ;10(10)Read more>>
Mouse brain lysates probed with Anti Cyclin M2 Polyclonal Antibody, Unconjugated (bs-6912R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cyclin M2 Antibody (bs-6912R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).