bs-6741R-Cy5 [Conjugated Primary Antibody]
ASC Polyclonal Antibody, Cy5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ASC

Immunogen Range: 31-130/195


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 29108

Swiss Prot: Q9ULZ3

Source: KLH conjugated synthetic peptide derived from human TMS1/ASC

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the DAPIN and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8.

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 22


Cross Reactive Species: Human
Mouse
Rat
Pig
Bacteria
Others

Predicted Cross Reactive Species: Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fan et al. Activation of Interleukin-1β Release by the Classical Swine Fever Virus Is Dependent on the NLRP3 Inflammasome, Which Affects Virus Growth in Monocytes. (2018) Front.Cell.Infect.Microbiol. 8:225Read more>>
  • Lee DW et al. Catheter-based renal sympathetic denervation induces acute renal inflammation through activation of caspase-1 and NLRP3 inflammasome. Anatol J Cardiol. 2019 Mar;21(3):134-141. Read more>>
  • Wang Y et al. Pre-administration of luteoline attenuates neonatal sevoflurane-induced neurotoxicity in mice. Acta Histochem. 2019 May;121(4):500-507.Read more>>
  • Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182.Read more>>
  • Wang Z et al. Secoisolariciresinol diglucoside suppresses Dextran sulfate sodium salt-induced colitis through inhibiting NLRP1 inflammasome. Int Immunopharmacol. 2020 Jan;78:105931.Read more>>
  • He S et al. GLI1 mediated pulmonary artery smooth muscle cell pyroptosis contributes to hypoxia induced pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 2019 Dec 23.Read more>>
  • Yang X et al. HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2. Int Immunopharmacol. 2020 Mar 16;83:106383.Read more>>
  • Yang X et al. HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2. Int Immunopharmacol. 2020 Mar 16;83:106383.Read more>>
  • Huang Y et al. Hypoxia-preconditioned olfactory mucosa mesenchymal stem cells abolish cerebral ischemia/reperfusion-induced pyroptosis and apoptotic death of microglial cells by activating HIF-1. Aging (Albany NY). 2020 Jun 7;12(11):10931-10950.Read more>>
  • Guan L et al. Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasome. Life Sci. 2020 Sep 1;256:117935.Read more>>
  • Shu-Min Yeet al. Silencing of Gasdermin D by siRNA-Loaded PEI-Chol Lipopolymers Potently Relieves Acute Gouty Arthritis through Inhibiting Pyroptosis. Mol Pharm. 2020 Jul 6.Read more>>
  • Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240.Read more>>
  • Mengli Cui et al. NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury. Int Immunopharmacol. 2020 Jul;84:106492.Read more>>
  • Hanwen Li et al. P2Y 14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death Dis.2020 May 26;11(5):394.Read more>>
  • Zhu Hui-Zheng. et al. Xiaoyaosan Exerts Therapeutic Effects on the Colon of Chronic Restraint Stress Model Rats via the Regulation of Immunoinflammatory Activation Induced by the TLR4/NLRP3 Inflammasome Signaling Pathway. Evid-Based Compl Alt. 2021;2021:6673538Read more>>
  • Hongxing Li. et al. Lipopolysaccharide upregulates miR-132/212 in Hirschsprung-associated enterocolitis, facilitating pyroptosis by activating NLRP3 inflammasome via targeting Sirtuin 1 (SIRT1). Aging-Us. 2020 Sep 30; 12(18): 18588C18602Read more>>
  • Xie Lulu. et al. The Anti-Depressive Effects of Hesperidin and the Relative Mechanisms Based on the NLRP3 Inflammatory Signaling Pathway. Front Pharmacol. 2020 Aug;11:1251Read more>>
  • Guangrui Chai. et al. NLRP3 Blockade Suppresses Pro-Inflammatory and Pro-Angiogenic Cytokine Secretion in Diabetic Retinopathy. Diabet Metab Synd Ob. 2020; 13: 3047C3058Read more>>
  • Kun Hao. et al. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. Int J Biol Sci. 2020; 16(16): 3163C3173Read more>>
  • Yuan Jiang. et al. Circular RNA Calm4 Regulates Hypoxia-Induced Pulmonary Arterial Smooth Muscle Cells Pyroptosis via the Circ-Calm4/miR-124-3p/PDCD6 Axis. Arterioscl Throm Vas. ;0:ATVBAHA.120.315525Read more>>
  • Xiping Yang. et al. The piperine derivative HJ105 inhibits A1-42-induced neuroinflammation and oxidative damage via the Keap1-Nrf2-TXNIP axis. Phytomedicine. 2021 Apr;:153571Read more>>
  • Yanyan Liu. et al. MIF inhibitor ISO-1 alleviates severe acute pancreatitis-associated acute kidney injury by suppressing the NLRP3 inflammasome signaling pathway. Int Immunopharmacol. 2021 Jul;96:107555Read more>>
  • Yaru Huang. et al. Dexmedetomidine attenuates myocardial ischemia-reperfusion injury in vitro by inhibiting NLRP3 Inflammasome activation. Bmc Anesthesiol. 2021 Dec;21(1):1-12Read more>>
  • Mingying Han. et al. Verapamil inhibits early acute liver failure through suppressing the NLRP3 inflammasome pathway. 2021 May 24Read more>>
  • You Zhou. et al. Overexpression of lncRNA TUG1 Alleviates NLRP3 Inflammasome-Mediated Cardiomyocyte Pyroptosis Through Targeting the miR-186-5p/XIAP Axis in Coronary Microembolization-Induced Myocardial Damage. Front Immunol. 2021; 12: 637598Read more>>
  • Cheng-Cheng Wang. et al. Dietary EPA-Enriched Phospholipids Alleviate Chronic Stress and LPS-induced Depression- and Anxiety-Like Behaviour by Regulating Immunity and Neuroinflammation. 2021 Jul 04Read more>>
  • Chenyu Zhang. et al. The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease. Redox Biol. 2021 Nov;47:102134Read more>>
  • Yan Li. et al. Ginsenoside Rg1 ameliorates aging?induced liver fibrosis by inhibiting the NOX4/NLRP3 inflammasome in SAMP8 mice. Mol Med Rep. 2021 Nov;24(5):1-14Read more>>
  • Lixia Peng. et al. Cardioprotective activity of ethyl acetate extract of Cinnamomi Ramulus against myocardial ischemia/reperfusion injury in rats via inhibiting NLRP3 inflammasome activation and pyroptosis. Phytomedicine. 2021 Dec;93:153798Read more>>
  • Jie Ma. et al. The Effect of MaxiK Channel on Regulating the Activation of NLRP3 Inflammasome in Rats of Blast-induced Traumatic Brain Injury. Neuroscience. 2022 Feb;482:132Read more>>
  • Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-B signaling pathway. Neurochem Int. 2021 Nov;:105221Read more>>
  • Jianzhao Liao. et al. Endoplasmic Reticulum Stress Contributes to Copper-Induced Pyroptosis via Regulating the IRE1_-XBP1 Pathway in Pig Jejunal Epithelial Cells. J Agr Food Chem. 2022;70(4):12931303Read more>>
  • Qing, Cai. et al. Protective Effects of 18_-Glycyrrhetinic Acid on Neonatal Rats with Hyperoxia Exposure. Inflammation. 2022 Jan;:1-15Read more>>
  • Yu W. et al. Cochlear Marginal Cell Pyroptosis Is Induced by Cisplatin <i>via</i> NLRP3 Inflammasome Activation.. FRONT IMMUNOL. 2022 Apr;13:823439-823439Read more>>
  • Sun Huanhuan. et al. Sulforaphane Ameliorates Limb Ischemia/Reperfusion-Induced Muscular Injury in Mice by Inhibiting Pyroptosis and Autophagy via the Nrf2-ARE Pathway. EVID-BASED COMPL ALT. 2022;2022:4653864Read more>>
  • Liyuan Wu. et al. Oridonin alleviates kanamycin-related hearing loss by inhibiting NLRP3/caspase-1/gasdermin D-induced inflammasome activation and hair cell pyroptosis. MOL IMMUNOL. 2022 Sep;149:66Read more>>
  • Dengjin Chen. et al. IL-1_ induced by PRRSV co-infection inhibited CSFV C-strain proliferation via the TLR4/NF-_B/MAPK pathways and the NLRP3 inflammasome. VET MICROBIOL. 2022 Jul;:109513Read more>>
  • Rui Guo. et al. Ulinastatin attenuates spinal cord injury by targeting AMPK/NLRP3 signaling pathway. J CHEM NEUROANAT. 2022 Nov;125:102145Read more>>
  • Zhou, Lingling. et al. Intrauterine exposure to oxidative stress induces caspase-1-dependent enteric nerve cell pyroptosis. PEDIATR SURG INT. 2022 Aug;:1-13Read more>>
  • Cheng-Cheng Wang. et al. DHA-Enriched Phospholipids Exhibit Anti-Depressant Effects by Immune and Neuroendocrine Regulation in Mice: A Study on Dose- and Structure-Activity Relationship. MOL NUTR FOOD RES. 2022 Sep;:2200089Read more>>
  • Xuemei Jia. et al. The potential mechanism of huazhuojiedu decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation.. FRONT PHARMACOL. 2022 Oct;13:1033874-1033874Read more>>
  • Xu Yang. et al. T-2 Toxin Caused Mice Testicular Inflammation Injury via ROS-Mediated NLRP3 Inflammasome Activation. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Fei Luan. et al. Cardioprotective effect of cinnamaldehyde pretreatment on ischemia/ reperfusion injury via inhibiting NLRP3 inflammasome activation and gasdermin D mediated cardiomyocyte pyroptosis. CHEM-BIOL INTERACT. 2022 Dec;368:110245Read more>>
  • Guo-Ping Wang. et al. To Analyze the Mechanism of SalB Regulating SIRT1 to Inhibit NLRP3 and Its Ameliorative Effect on Tubulogastric Junction Tumor Lesions Complicated with Myocardial Injury. BIOMED RES INT. 2022 Oct 14;2022:6560693Read more>>
  • Shen Yanghui. et al. NLRP3 inflammasome inhibitor MCC950 can reduce the damage of pancreatic and intestinal barrier function in mice with acute pancreatitis. ACTA CIR BRAS. 2022 Oct;37Read more>>
  • Yan, Minghao. et al. Chronic Microcystin-LR-Induced -Synuclein Promotes Neuroinflammation Through Activation of the NLRP3 Inflammasome in Microglia. MOL NEUROBIOL. 2022 Nov;:1-17Read more>>
  • Hanzhou Li. et al. San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway.. J DIABETES RES. 2022 Nov;2022:2640209-2640209Read more>>
  • Li Jing. et al. DBDPE upregulates NOD-like receptor signaling to induce NLRP3 inflammasome-mediated HAECs pyroptosis. ENVIRON POLLUT. 2023 Feb;318:120882Read more>>
  • Yuying Chen. et al. Cadmium exposure promotes thyroid pyroptosis and endocrine dysfunction by inhibiting Nrf2/Keap1 signaling. ECOTOX ENVIRON SAFE. 2023 Jan;249:114376Read more>>
  • Peiran Cong. et al. Hydroxysafflor yellow A mitigates myocardial fibrosis induced by isoproterenol and angiotensin II. AM J TRANSL RES. 2022; 14(12): 8588C8598Read more>>
VALIDATION IMAGES