bs-6705R [Primary Antibody]
cGKI Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: cGKI

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5592

Source: KLH conjugated synthetic peptide derived from human Prkg1/cGKI

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PKG plays an important stimulatory role in platelet activation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
ICC/IF(ICC/IF=1:100)

Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huan X et al. PDE9 inhibition promotes proliferation of neural stem cells via cGMP-PKG pathway following oxygen-glucose deprivation/reoxygenation injury in vitro. Neurochem Int. 2020 Feb;133:104630. Read more>>
  • Huan Xiao. et al. Gastrodin promotes hippocampal neurogenesis via PDE9-cGMP-PKG pathway in mice following cerebral ischemia. Neurochem Int. 2021 Nov;150:105171Read more>>
  • Liu, Yiwei. et al. An electrochemical nitric oxide generator for in-home inhalation therapy in pulmonary artery hypertension. BMC MED. 2022 Dec;20(1):1-13Read more>>
VALIDATION IMAGES

Lane 1: heart lysates; Lane 2: ceretrum lysates probed with cGKI Polyclonal Antibody, Unconjugated (bs-6705R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.