Host: Rabbit
Target Protein: Calpastatin
Immunogen Range: 431-530/708
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 831
Swiss Prot: P20810
Source: KLH conjugated synthetic peptide derived from human Calpastatin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 70
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Huang, Zhi, et al. "Calpastatin Prevents NF-κB–Mediated Hyperactivation of Macrophages and Attenuates Colitis." The Journal of Immunology (2013).Read more>>
Human Raji cells probed with calpastatin Polyclonal Antibody, unconjugated (bs-6701R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse lung lysates probed with Calpastatin Polyclonal Antibody, unconjugated (bs-6701R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpastatin Polyclonal Antibody, Unconjugated (bs-6701R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpastatin Polyclonal Antibody, Unconjugated (bs-6701R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human MCF-7 cell lysates probed with Calpastatin Polyclonal Antibody, Unconjugated (bs-6701R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calpastatin Polyclonal Antibody, Unconjugated (bs-6701R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.