DATASHEET
Host:
Rabbit
Target Protein:
KCNN4
Immunogen Range:
325-427/427
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3783
Swiss Prot:
O15554
Source:
KLH conjugated synthetic peptide derived from human KCNN4
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang, Panshi, et al. "Inhibition of SK4 Potassium Channels Suppresses Cell Proliferation, Migration and the Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells." PLOS ONE 11.4 (2016): e0154471.Read more>>
- Lively et al. Comparing Effects of Transforming Growth Factor β1 on Microglia From Rat and Mouse: Transcriptional Profiles and Potassium Channels. (2018) Front.Cell.Neurosci. 12:115Read more>>
- Liu Dishiwen. et al. Cardiac Fibroblasts Promote Ferroptosis in Atrial Fibrillation by Secreting Exo-miR-23a-3p Targeting SLC7A11. OXID MED CELL LONGEV. 2022;2022:3961495Read more>>
VALIDATION IMAGES
Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 50kD. Observed band size: 45kD, 50kD.
Formalin-fixed and paraffin embedded rat brain labeled with Anti KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U-2 OS cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human LOVO cell lysates; Lane 6: Human HepG2 cell lysates probed with KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.