bs-6663R [Primary Antibody]
HRH1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HRH1

Immunogen Range: 141-240/487


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3269

Swiss Prot: P35367

Source: KLH conjugated synthetic peptide derived from human HRH1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 54


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Møller, M. Nue, et al. "Expression of histamine receptors in the human endolymphatic sac: the molecular rationale for betahistine use in Menieres disease." European Archives of Oto-Rhino-Laryngology: 1-6.Read more>>
  • Girodet et al. Alternative Macrophage Activation Is Increased in Asthma. (2016) Am.J.Respir.Cell.Mol.Biol. 55:467-475Read more>>
  • Aya Sato. et al. Histamine depolarizes rat intracardiac ganglion neurons through the activation of TRPC non-selective cation channels. Eur J Pharmacol. 2020 Nov;886:173536Read more>>
  • Liao, Xiaodan. et al. Fullerene nanoparticles for the treatment of ulcerative colitis. 2021 Nov 02Read more>>
  • Kristine Elisabeth Eberhard. et al. Neurotransmitter and Neurotransmitter Receptor Expression in the Saccule of the Human Vestibular System. Prog Neurobiol. 2022 Jan;:102238Read more>>
  • Yu Wen-Yan. et al. Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation. Evid-Based Compl Alt. 2022;2022:4571981Read more>>
VALIDATION IMAGES

Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human Hela cell lysates probed with HRH1 Polyclonal Antibody, Unconjugated (bs-6663R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.