bs-6655R
[Primary Antibody]
Cryopyrin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Cryopyrin
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 114548
Source: KLH conjugated synthetic peptide derived from human Cryopyrin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
Size: 100ul
Concentration: 1ug/ul
Applications:
FCM(1:20-100)
IHC-P(1:200-400)
Cross Reactive Species:
Human
Mouse
Rat
Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhao J et al. Cepharanthine attenuates cerebral ischemia/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12/15-LOX signaling. Biomed Pharmacother. 2020 Jul;127:110151.Read more>>
- Chen Y et al. Dendritic cells-derived interferon-1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414.Read more>>
- Shu-Min Yeet al. Silencing of Gasdermin D by siRNA-Loaded PEI-Chol Lipopolymers Potently Relieves Acute Gouty Arthritis through Inhibiting Pyroptosis. Mol Pharm. 2020 Jul 6.Read more>>
- Xianzhen Zhang. et al. Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL?1 pathway. Mol Med Rep. 2021 Mar;23(3):1-1Read more>>
- Kun Hao. et al. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. Int J Biol Sci. 2020; 16(16): 3163C3173Read more>>
- Runxiao Zheng. et al. Particulate matter aggravates Alzheimer's disease by activating the NLRP3 inflammasome to release ASC specks. Environ Sci-Nano. 2021 JuRead more>>
- Xiaohong Xu. et al. Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-B/NLRP3 inflammation pathway. Bmc Nephrol. 2021 Dec;22(1):1-17Read more>>
- Wenwen Liu. et al. Effects of andrographolide on renal tubulointersticial injury and fibrosis. Evidence of its mechanism of action. Phytomedicine. 2021 Jul;:15365Read more>>
- Yitong Pan. et al. N-acetyl-L-tryptophan attenuates hepatic ischemia-reperfusion injury via regulating TLR4/NLRP3 signaling pathway in rats. Peerj. 2021 Aug;9:e11909Read more>>
- Wei Zhou. et al. Computational Systems Pharmacology, Molecular Docking and Experiments Reveal the Protective Mechanism of Li-Da-Qian Mixture in the Treatment of Glomerulonephritis. J Inflamm Res. 2021 Dec;14:6939-6958Read more>>
- Shuaitao Yang. et al. Blockage of transient receptor potential vanilloid 4 prevents postoperative atrial fibrillation by inhibiting NLRP3-inflammasome in sterile pericarditis mice. CELL CALCIUM. Cell Calcium. 2022 Apr;:10259Read more>>
- Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078Read more>>
- Song Xue. et al. Receptor-Interacting Protein Kinase 3 Inhibition Relieves Mechanical Allodynia and Suppresses NLRP3 Inflammasome and NF-_B in a Rat Model of Spinal Cord Injury. FRONT MOL NEUROSCI. 2022; 15: 861312Read more>>
- Zhang Haoyun. et al. Spatiotemporal evolution of pyroptosis and canonical inflammasome pathway in hSOD1G93A ALS mouse model. BMC NEUROSCI. 2022 Dec;23(1):1-14Read more>>
- Zineng Yan. et al. Engineering exosomes by three-dimensional porous scaffold culture of human umbilical cord mesenchymal stem cells promotes osteochondral repair. MATER TODAY BIO. 2023 Jan;:100549Read more>>
- Xi-min Hu. et al. Pyroptosis-related gene signature elicits immune response in rosacea. EXP DERMATOL. 2023 ApRead more>>
- Yu Zhang. et al. Blocking CXC Motif Chemokine Ligand 2 Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Apoptosis and NLRP3 Inflammasome Activation. BIOL PHARM BULL. 2023 May;46(5):672-683Read more>>
- Jirui Lang. et al. LC-MS-based metabolomics reveals the mechanism of anti-gouty arthritis effect of Wuwei Shexiang pill. FRONT PHARMACOL. 2023; 14: 1213602Read more>>
- Chengjie Wu. et al. Inhibition of HDAC6 promotes microvascular endothelial cells to phagocytize myelin debris and reduces inflammatory response to accelerate the repair of spinal cord injury. CNS NEUROSCI THER. 2023 AugRead more>>
- Xiaoli Chen. et al. HET0016 inhibits neuronal pyroptosis in the immature brain post-TBI via the p38 MAPK signaling pathway. NEUROPHARMACOLOGY. 2023 Nov;239:109687Read more>>
- Xi-min Hu. et al. PANoptosis signaling enables broad immune response in psoriasis: From pathogenesis to new therapeutic strategies. COMPUT STRUCT BIOTEC. 2024 Dec;23:64Read more>>
- Tietao Di. et al. Emodin Blocks mPTP Opening and Improves LPS-induced HMEC-1Cell Injury by Upregulation of ATP5A1. CHEM BIODIVERS. 2024 Mar;:e202301916Read more>>
- Qifang Sun. et al. Astragaloside IV ameliorates indomethacin-induced intestinal inflammation in rats through inhibiting the activation of NLRP3 inflammasome. INT IMMUNOPHARMACOL. 2024 Jun;135:112281Read more>>
- Zhou Xinghong. et al. Gut microbiota dysbiosis in hyperuricaemia promotes renal injury through the activation of NLRP3 inflammasome. MICROBIOME. 2024 Dec;12(1):1-2Read more>>
- Chengzhi Yuan. et al. Atractylenolide I Alleviates Indomethacin-Induced Gastric Ulcers in Rats by Inhibiting NLRP3 Inflammasome Activation. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES
RAW264.7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NALP3/CIAS1 Antibody(bs-6655R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic inflammatory mastitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.