DATASHEET
Host:
Rabbit
Target Protein:
WNT7A
Immunogen Range:
241-349/349
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
7476
Swiss Prot:
O00755
Source:
KLH conjugated synthetic peptide derived from human WNT7A
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts (By similarity).
PRODUCT SPECIFIC PUBLICATIONS
- ZHANG P et al. 5-Azacytidine and trichostatin A enhance the osteogenic differentiation of bone marrow mesenchymal stem cells isolated from steroid-induced avascular necrosis of the femoral head in rabbit. J Biosci (2019) 44:87.Read more>>
- Jianglin Wang. et al. Validation and analysis of expression, prognosis and immune infiltration of WNT gene family in non-small cell lung cancer. FRONT ONCOL. 2022; 12: 911316Read more>>
VALIDATION IMAGES
Mouse brain lysates probed with Rabbit Anti-WNT7A Polyclonal Antibody, Unconjugated (bs-6645R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Mouse Kidney tissue lysates ; Lane 5: Rat Placenta tissue lysates; Lane 6: Rat Lung tissue lysates; Lane 7: Rat Testis tissue lysates ; Lane 8: Rat Kidney tissue lysates ; Lane 9: Human Hela cell lysates ; Lane 10: Human A431 cell lysates ; Lane 11: Human SH-SY5Y cell lysates ; Lane 12: Human HepG2 cell lysates probed with WNT7A Polyclonal Antibody, Unconjugated (bs-6645R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.