Host: Rabbit
Target Protein: Vinculin
Immunogen Range: 1001-1134/1134
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7414
Swiss Prot: P18206
Source: KLH conjugated synthetic peptide derived from human Vinculin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 125
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
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Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vinculin Polyclonal Antibody, Unconjugated (bs-6640R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.