DATASHEET
Host:
Rabbit
Target Protein:
PAPPA
Immunogen Range:
1321-1460/1627
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5069
Swiss Prot:
Q13219
Source:
KLH conjugated synthetic peptide derived from human PAPPA
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
PRODUCT SPECIFIC PUBLICATIONS
- Li, Jiaxun, et al. "Competitive detection of pregnancy-associated plasma protein-A in serum using functional single walled carbon nanotubes/chitosan-based electrochemical immunosensor." Journal of Electroanalytical Chemistry (2013).Read more>>
- Kannan, Athilakshmi. et al. Maternal high-fat diet during pregnancy with concurrent phthalate exposure leads?to abnormal placentation. Sci Rep-Uk. 2021 Aug;11(1):1-12Read more>>
- Jing Liu. et al. Exosomes Promote Atherosclerosis Progression by Regulating Circ_100696/miR-503-5p/PAPPA Axis-Mediated Vascular Smooth Muscle Cells Proliferation and Migration. INT HEART J. 2023 Sep;64(5):918-927Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti PAPPA Polyclonal Antibody, Unconjugated (bs-6618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-6618P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450