Host: Rabbit
Target Protein: Podocin
Immunogen Range: 231-330/383
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7827
Swiss Prot: Q9NP85
Source: KLH conjugated synthetic peptide derived from human Podocin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Zhang, Xiao-Liang, et al. "Vitamin D Prevents Podocyte Injury Via Regulation Of Macrophage M1/M2 Phenotype In Diabetic Nephropathy Rats." Endocrinology (2014).Read more>>
- Jayachandran, Muthuvel, et al. "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." Biol Sex Differ 6.2 (2015).Read more>>
- Garovic, Vesna D., and Muthuvel Jayachandran. "DETECTING PODOCYTE INJURY IN DIABETIC NEPHROPATHY AND GLOMERULONEPHRITIS." U.S. Patent No. 20,170,003,299. 5 Jan. 2017.Read more>>
- Ishida et al. Kidney-specific Sonoporation-mediated Gene Transfer. (2016) Mol.The. 24:125-34Read more>>
- Turco et al. Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys. (2016) J.Extracell.Vesicle. 5:29642Read more>>
- Maeda et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. (2018) J.Clin.Invest. 128:3445-3459Read more>>
- Maeda et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. (2018) J.Clin.Invest. 128:3445-3459Read more>>
- Cao S et al. Effect of shenyan xiaobai granule on nephrin and podocin of adriamycin-induced renal injury: A randomised controlled trial. J Ethnopharmacol. 2019 Nov 15;244:112104. Read more>>
- Fang-Hao Cai. et al. Diagnostic roles of urinary kidney microvesicles in diabetic nephropathy. Ann Transl Med. 2020 Nov; 8(21): 1431Read more>>
- Alberto Mella. et al. Detection of urinary podocytes by flow cytometry in idiopathic membranous nephropathy. Sci Rep-Uk. 2020 Oct;10(1):1-7Read more>>
- Nan Zang. et al. cGAS-STING Activation by Cytosolic Mitochondrial DNA Contributes to Podocyte Injury in Diabetic Kidney Disease. ISCIENCE. 2022 Sep;:105145Read more>>
- Mohani Chandra Irwanadi. et al. DLBS3233 enhances nephrin and podocin expression also reduces oxidative stress marker and insulin receptor serine diabetic rats podocytes. ANN MED SURG. 2023 Mar;:10.1097/MS9.0000000000000308Read more>>
- Shin Young Kim. et al. Characterizing Glomerular Barrier Dysfunction with Patient-Derived Serum in Glomerulus-on-a-Chip Models: Unveiling New Insights into Glomerulonephritis. INT J MOL SCI. 2024 Jan;25(10):5121Read more>>
- J.I. Yue. et al. Acupuncture improve proteinuria in diabetic kidney disease rats by inhibiting ferroptosis and epithelial-mesenchymal transition. HELIYON. 2024 Jul;1Read more>>
Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPHS2) Polyclonal Antibody, Unconjugated (bs-6597R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. \n)\n
Mouse kidney lysates probed with Podocin Polyclonal Antibody, unconjugated (bs-6597R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates; Lane 3: Human 293T cell lysates; Lane 4: Human Jurkat cell lysates probed with NPHS2 Polyclonal Antibody, Unconjugated (bs-6597R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Polyclonal Antibody, Unconjugated (bs-6597R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Polyclonal Antibody, Unconjugated (bs-6597R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with NPHS2 polyclonal antibody, unconjugated (bs-6597R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.