DATASHEET
Host:
Rabbit
Target Protein:
S100A15
Immunogen Range:
41-108/108
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
381493
Swiss Prot:
Q6S5I3
Source:
KLH conjugated synthetic peptide derived from mouse S100A15
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients.
PRODUCT SPECIFIC PUBLICATIONS
- Hau, Carren S., et al. "Visfatin Enhances the Production of Cathelicidin Antimicrobial Peptide, Human ??-Defensin-2, Human ??-Defensin-3, and S100A7 in Human Keratinocytes and Their Orthologs in Murine Imiquimod-Induced Psoriatic Skin."he American Journal of Pathology (2013)..Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-S100A7 Polyclonal Antibody, Unconjugated(bs-6575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with S100A7 Antibody(bs-6575R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).