bs-6505R-Cy3 [Conjugated Primary Antibody]
15 Lipoxygenase 1 Polyclonal Antibody, Cy3 Conjugated
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Host: Rabbit

Target Protein: 15 Lipoxygenase 1

Immunogen Range: 581-662/662


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 246

Swiss Prot: P16050

Source: KLH conjugated synthetic peptide derived from human ALOX15/15 Lipoxygenase 1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)

Predicted Molecular Weight: 73


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Francos‐Quijorna, Isaac, et al. "IL‐4 drives microglia and macrophages toward a phenotype conducive for tissue repair and functional recovery after spinal cord injury." Glia (2016).Read more>>
  • Dalli, Jesmond, et al. "Vagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution." Immunity (2017).Read more>>
  • Dalli et al. Vagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution. (2017) Immunity. 46:92-105Read more>>
  • Dalli et al. Vagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution. (2017) Immunity. 46:92-105Read more>>
  • Takuya Kuzumoto. et al. Protective role of resolvin D1, a pro-resolving lipid mediator, in nonsteroidal anti-inflammatory drug-induced small intestinal damage. Plos One. 2021 May;16(5):e0250862Read more>>
  • Raquel M. Marques. et al. Loss of 15-lipoxygenase disrupts Treg differentiation altering their pro-resolving functions. Cell Death Differ. 2021 May;:1-21Read more>>
  • ennan D. Gerlach. et al. Efferocytosis induces macrophage proliferation to help resolve tissue injury. Cell Metab. 2021 Dec;33:2445Read more>>
  • Hui Wang. et al. Effect of Chlorogenic Acid via Upregulating Resolvin D1 Inhibiting the NF-B Pathway on Chronic Restraint Stress-Induced Liver Inflammation. J AGR FOOD CHEM. 2022;70(34):1053210542Read more>>
  • Pier Giorgio Puzzovio. et al. Mast cells contribute to the resolution of allergic inflammation by releasing resolvin D1. PHARMACOL RES. 2023 Mar;189:106691Read more>>
  • Duco Steven Koenis. et al. Efferocyte-Derived MCTRs Metabolically Prime Macrophages for Continual Efferocytosis via Rac1-Mediated Activation of Glycolysis. ADV SCI. 2023 Dec;:230469Read more>>