bs-6472R [Primary Antibody]
Phospholipase C beta 2 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Phospholipase C beta 2

Immunogen Range: 401-500/1185


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5330

Swiss Prot: Q00722

Source: KLH conjugated synthetic peptide derived from human Phospholipase C beta 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 130


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HeLa cells probed with Phospholipase C beta 2 Antibody, ALEXA FLUOR® 647 Conjugated (bs-6472R-A647) at 1:100 for 30 minutes compared to control cells (blue)and isotype control (orange).


HeLa cells probed with Phospholipase C beta 2 Antibody, ALEXA FLUOR® 647 Conjugated (bs-6472R-A647) at 1:100 for 30 minutes compared to control cells (blue)and isotype control (orange).


HL60 cell lysates probed with Phospholipase C beta 2 Polyclonal Antibody, Unconjugated (bs-6472R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.