DATASHEET
Host:
Rabbit
Target Protein:
PRLR
Immunogen Range:
251-350/622
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5618
Swiss Prot:
P16471
Source:
KLH conjugated synthetic peptide derived from human Prolactin Receptor
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
PRODUCT SPECIFIC PUBLICATIONS
- Xie W et al. Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus). Eur J Histochem. 2019 Jan 17;63(1). Read more>>
- Yang Yu et al. Vaccarin promotes proliferation of and milk synthesis in bovine mammary epithelial cells through the Prl receptor-PI3K signaling pathway. Eur J Pharmacol. 2020 Aug 5;880:173190.Read more>>
- Yongfu La. et al. Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak. 2021 Nov 09Read more>>
- Senta Georgia. et al. Exogenous Lactogenic Signaling Stimulates Beta Cell Replication In Vivo and In Vitro. Biomolecules. 2022 Feb;12(2):215Read more>>
VALIDATION IMAGES
Lane 1: mouse brain lysates probed with Rabbit Anti-PRLR Polyclonal Antibody, Unconjugated (bs-6445R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti-PRLR Polyclonal Antibody, Unconjugated (bs-6445R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with PRLR Polyclonal Antibody (bs-6445R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.