Host: Rabbit
Target Protein: ACP5
Immunogen Range: 101-200/325
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 54
Swiss Prot: P13686
Source: KLH conjugated synthetic peptide derived from human Tartrate-resistant acid phosphatase type 5
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
Predicted Molecular Weight: 34
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Sun, Weijia, et al. "Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity." Cell Discovery 2 (2016).Read more>>
- Adem Tokpinar. et al. The effect of ionizing radiation on the fetal bone development in pregnant rats: Role of melatonin. MICROSC RES TECHNIQ. 2023 SepRead more>>
Molt-4 cell lysates probed with Tartrate Resistant Acid Phosphatase Polyclonal Antibody, Unconjugated (bs-6434R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver; Probed with Tartrate Resistant Acid Phosphatase Polyclonal Antibody, Unconjugated (bs-6434R) at 1:300, overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ACP5 Antibody(bs-6434R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).