bs-6425R [Primary Antibody]
TRPV4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TRPV4

Immunogen Range: 301-400/871


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 59341

Swiss Prot: Q9HBA0

Source: KLH conjugated synthetic peptide derived from human TRPV4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Flow-Cyt(Flow-Cyt=1ug/test)
ICC/IF(ICC/IF=1:100)

Predicted Molecular Weight: 96


Cross Reactive Species: Human
Bovine

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sakakibara A, Sakakibara S, Kusumoto J, Takeda D, Hasegawa T, Akashi M, et al. (2017) Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking. PLoS ONE 12(1): e0169723.Read more>>
  • Walter et al. Reduced tissue osmolarity increases TRPV4 expression and pro-inflammatory cytokines in intervertebral disc cells. (2016) Eur.Cell.Mater. 32:123-36Read more>>
  • Ghoneum, M.H., et al. “Inhibition of TRPV1 Channel Activity in Human CD4⁺ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576.” Nanomaterials (2018); 8(10): E770.Read more>>
  • Ghoneum et al. Inhibition of TRPV1 Channel Activity in Human CD4⁺ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576. (2018) Nanomaterials.(Basel). 8 Read more>>
  • Ge Ling Ying. et al. Management of experimental trabeculectomy filtering blebs via crosslinking of the scleral flap inhibited vascularization. GRAEF ARCH CLIN EXP. 2023 Nov;:1-11Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRP12 Polyclonal Antibody, Unconjugated (bs-6425R) at 1:400 overnight at 4°C, DAB staining.