bs-6417R [Primary Antibody]
PI3 Kinase p110 beta(Ser1070) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PI3 Kinase p110 beta Ser1070

Specificity: This phosphorylation site is homologous to that of Ser1064 in Mouse and Ser1070 in Rat.

Modification Site: Ser1070

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5291

Swiss Prot: P42338

Source: KLH conjugated synthetic phosphopeptide derived from human PI3 Kinase p110 beta around the phosphorylation site of Ser1070

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 110-123


Cross Reactive Species: Human
Mouse
Rat
Dog
Rabbit

Predicted Cross Reactive Species: Cow
Pig
Horse
Chicken
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qiao, Jingwen, et al. "Involvement of Akt/GSK3β/CREB signaling pathway on chronic omethoate induced depressive-like behavior and improvement effects of combined lithium chloride and astaxanthin treatment." Neuroscience Letters (2017).Read more>>
  • Li, Zhao, et al. "The aqueous extract of Curcuma wenyujin rescues learning and memory deficits through PI3k/Akt/GSK-3β pathway in Aβ-induced AD mice." Biomedical Research 28.17 (2017): 7438-7442.Read more>>
  • Liu,et al.Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.(2018) Angiogenesis. 21:237-249.Read more>>
  • Wang,et al.FOXD3/FOXD4 is required for the development of hindgut in the rat model of anorectal malformation.(2018) Experimental Biology and Medicine. 243:327-333.Read more>>
  • Li B et al. Resistin up-regulates LPL expression through the PPARγ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264. 7 macrophages.Cytokine. 2019 Jul;119:168-174.Read more>>
  • Li B et al. Resistin up-regulates LPL expression through the PPARγ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264. 7 macrophages.Cytokine. 2019 Jul;119:168-174.Read more>>
  • Wang Z et al. Interaction between Endothelin-1 and Left Stellate Ganglion Activation: A Potential Mechanism of Malignant Ventricular Arrhythmia during Myocardial Ischemia. Oxid Med Cell Longev. 2019 May 12;2019:6508328. Read more>>
  • Yang G et al. Ginsenoside Rb1 Prevents Dysfunction of Endothelial Cells by Suppressing Inflammatory Response and Apoptosis in the High-Fat Diet Plus Balloon Catheter-Injured Rabbit Model via the G Protein-Coupled Estrogen Receptor-Mediated Phosphatidylinositol 3-Kinases (PI3K)/Akt Pathway. Med Sci Monit. 2019 Oct 3;25:7407-7417.Read more>>
  • Yang RP et al. Pharmacological actions of neferine in the modulation of human platelet function. Eur J Pharmacol. 2019 Aug 21:172626. Read more>>
  • Jin Y et al. miR‐496 remedies hypoxia reoxygenation–induced H9c2 cardiomyocyte apoptosis via Hook3‐targeted PI3k/Akt/mTOR signaling pathway activation. J Cell Biochem. 2019 Aug 22. Read more>>
  • Chen Y et al. Epothilone B prevents lipopolysaccharide-induced inflammatory osteolysis through suppressing osteoclastogenesis via STAT3 signaling pathway. Aging (Albany NY). 2020 Jun 11;12(12):11698-11716.Read more>>
  • Liu X et al. miR\34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ\8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways. J Cell Physiol. 2020 Dec;235(12):10051-10067.Read more>>
  • Zhang Y et al. A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development. Front Cell Dev Biol. 2020 Jun 16;8:383.Read more>>
  • Jianwen Longet al. Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3K/Akt/mTOR Signaling Pathway. Biomed Res Int. 2020 Jul 17;2020:5149417.Read more>>
  • Shujuan Liuet al. Pou2F3 silencing enhanced the proliferation of mammary epithelial cells in dairy Goat via PI3K/AKT/mTOR signaling pathway. Anim Biotechnol. 2020 Jul 30;1-9.Read more>>
  • Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
  • Han Chen. et al. ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-B signaling pathway. Bmc Cancer. 2020 Dec;20(1):1-1Read more>>
  • Yin Zhuang. et al. Exosomes Secreted by Nucleus Pulposus Stem Cells Derived From Degenerative Intervertebral Disc Exacerbate Annulus Fibrosus Cell Degradation via Let-7b-5p. Front Mol Biosci. 2021; 8: 766115Read more>>
  • Siyuan Liu. et al. _-Hydroxybutyrate impairs the release of bovine neutrophil extracellular traps through inhibiting phosphoinositide 3-kinasemediated nicotinamide adenine dinucleotide phosphate oxidase reactive oxygen species production. J Dairy Sci. 2022 FebRead more>>
  • Li Ma. et al. Effect of MiR-100-5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2022 Apr 12Read more>>
  • Chen Feng. et al. Xiao-Chai-Hu Decoction Ameliorates Poly (I:C)-Induced Viral Pneumonia through Inhibiting Inflammatory Response and Modulating Serum Metabolism. EVID-BASED COMPL ALT. 2022;2022:1240242Read more>>
  • Dongfang Lv. et al. Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.. FRONT PHARMACOL. 2022 Sep;13:961011-961011Read more>>
  • Wenyan Guo. et al. Effect of thymoquinone on sepsis-induced cardiac damage via anti-inflammatory and anti-apoptotic mechanisms. J INT MED RES. ;()Read more>>
  • Yu Ma. et al. Network pharmacology analysis combined with experimental validation to explore the therapeutic mechanism of Schisandra Chinensis Mixture on diabetic nephropathy. J ETHNOPHARMACOL. 2023 Feb;302:115768Read more>>
  • Xiaoyu Sui. et al. hsa-mir-133a-2 promotes the proliferation and invasion of cervical cancer cells by targeting the LAMB3-mediated PI3K/ATK pathway. CANCER MED-US. 2022 OcRead more>>
  • Shugen Xu. et al. Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringers solution. ANN TRANSL MED. 2022 Dec; 10(24): 1331Read more>>
  • Yanhong Pei. et al. Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury. MATER TODAY ADV. 2023 Mar;17:100349Read more>>
  • Chenglin Li. et al. Ranitidine as an adjuvant regulates macrophage polarization and activates CTLs through the PI3K-Akt2 signaling pathway. INT IMMUNOPHARMACOL. 2023 Mar;116:109729Read more>>
  • Xinyang Fan. et al. CEBPA-Regulated Expression of SOCS1 Suppresses Milk Protein Synthesis through mTOR and JAK2-STAT5 Signaling Pathways in Buffalo Mammary Epithelial Cells. FOODS. 2023 Jan;12(4):708Read more>>
  • Li-You Chen. et al. Luteolin improves nephropathy in hyperglycemic rats through anti-oxidant, anti-inflammatory, and anti-apoptotic mechanisms. J FUNCT FOODS. 2023 Mar;102:105461Read more>>
  • Xinyang Fan. et al. MiR-190a regulates milk protein biosynthesis through the mTOR and JAK2CSTAT5 signaling pathways by targeting PTHLH in buffalo mammary epithelial cells. J FUNCT FOODS. 2023 Mar;102:105451Read more>>
  • Fei Chen. et al. Antitumor mechanism of kangliu pill on gliomas in mice through PI3K-Akt signaling pathway. J ETHNOPHARMACOL. 2023 Feb;:116252Read more>>
  • Xiya Guo. et al. The ALK1?Smad1/5?ID1 pathway participates in tumour angiogenesis induced by low?dose photodynamic therapy. INT J ONCOL. 2023 Apr;62(4):1-16Read more>>
  • Shaogeng Chen. et al. PHLDA3 activated by BARX2 transcription, suppresses the malignant development of esophageal squamous cell carcinoma by downregulating PI3K/AKT levels. EXP CELL RES. 2023 Mar;:113567Read more>>
  • Tingting Zhao. et al. Yishen Huashi granule modulated lipid metabolism in diabetic nephropathy via PI3K/AKT/mTOR signaling pathways.. HELIYON. 2023 Mar;9(3):e14171-e14171Read more>>
  • Li Li. et al. Regulation of TREM2 on BV2 inflammation through PI3K/AKT/mTOR pathway. BIOTECHNOL GENET ENG. 2023 Apr 26Read more>>
  • Qiao Wanchen. et al. Liensinine ameliorates ischemiaCreperfusion-induced brain injury by inhibiting autophagy via PI3K/AKT signaling. FUNCT INTEGR GENOMIC. 2023 Jun;23(2):1-9Read more>>
  • Yun Chen. et al. Extracellular vesicles derived from Akkermansia muciniphila promote placentation and mitigate preeclampsia in a mouse model. J EXTRACELL VESICLES. 2023 May;12(5):12328Read more>>
  • Yue Zhang. et al. Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland. DEV BIOL. 2023 MayRead more>>
  • Jie Xu. et al. PI3K-AKT-mTOR signaling pathway regulates autophagy of hippocampal neurons in diabetic rats with chronic unpredictable mild stress. BEHAV BRAIN RES. 2023 Aug;452:114558Read more>>
  • Jing Li. et al. Hepatoprotective efficacy and interventional mechanism of JianPi LiShi YangGan formula in acute-on-chronic liver failure. J ETHNOPHARMACOL. 2023 Jul;:11688Read more>>
  • Zhenjie Wang. et al. Effect of sodium bicarbonate Ringer's solution on lung injury in rats with traumatic hemorrhagic shock. J BIOCHEM MOL TOXIC. 2023 Dec;:e23608Read more>>
  • Xin Miao. et al. AAV-mediated VEGFA overexpression promotes angiogenesis and recovery of locomotor function following spinal cord injury via PI3K/Akt signaling. EXP NEUROL. 2024 May;375:114739Read more>>
  • Qiushi Xu. et al. Role of diacylglycerol O-acyltransferase 1 (DGAT1) in lipolysis and autophagy of adipose tissue from ketotic dairy cows. J DAIRY SCI. 2024 FebRead more>>
  • Mingli An. et al. PI3K/AKT signaling pathway associates with pyroptosis and inflammation in patients with endometriosis. J REPROD IMMUNOL. 2024 Mar;162:104213Read more>>
  • Kaina Xu. et al. BMSCs attenuate radiation-induced brain injury induced hippocampal neuronal apoptosis through a PI3K/Akt/Bax/Bcl-2 signaling pathway. BRAIN RES. 2024 Apr;1829:148795Read more>>
  • Sheng Hu. et al. Ginsenoside Rd enhances blood-brain barrier integrity after cerebral ischemia/reperfusion by alleviating endothelial cells ferroptosis via activation of NRG1/ErbB4-mediated PI3K/Akt/mTOR signaling pathway. NEUROPHARMACOLOGY. 2024 Jun;251:109929Read more>>
  • Liang Huang. et al. Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K/AKT/mTOR signaling pathways. POULTRY SCI. 2024 Mar;:103696Read more>>
  • Hao-ru Guan. et al. Exploring the efficacy and mechanism of Bailing capsule to improve polycystic ovary syndrome in mice based on intestinal-derived LPS-TLR4 pathway. J ETHNOPHARMACOL. 2024 Apr;:118274Read more>>
  • Zhou Qixiu. et al. Mechanism of action of Taohong Siwu decoction in the alleviation of primary dysmenorrhea. FRONT MED-LAUSANNE. 2024 Apr;11Read more>>
VALIDATION IMAGES

Lane 1: human colon carcinoma lysates Lane 2: mouse brain lysates probed with Anti phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 110-123kD. Observed band size: 115,120kD.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Lane 1: Human MCF-7 cell lysates; Lane 2: Human K562 cell lysates probed with phospho-PI3 Kinase p110 beta (Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.