bs-6416R [Primary Antibody]
Complement fragment 3c Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Complement fragment 3c

Immunogen Range: 1051-1200/1663


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 718

Swiss Prot: P01024

Source: KLH conjugated synthetic peptide derived from human Complement C3c alpha' chain fragment 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:837664, PubMed:29953, PubMed:959512, PubMed:1432298, PubMed:15833747, PubMed:16333141, PubMed:1961575).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 183


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Vass, A., et al. "Inflammatory Activation after Experimental Cardiac Tamponade." European Surgical Research 51.1-2 (2013): 1-13.Read more>>
  • Ehrnthaller C et al. Hemorrhagic shock induces renal complement activation. Eur J Trauma Emerg Surg. 2019 Jul 15. Read more>>
  • Liu, Tao. et al. Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther. 2021 Dec;23(1):1-15Read more>>
  • Peng Zhang. et al. Dioscin ameliorates silica-aggravated systemic lupus erythematosus via suppressing apoptosis and improving LC3-associated phagocytosis in MRL/lpr mice. INT IMMUNOPHARMACOL. 2023 Mar;116:109814Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat lung labeled with Rabbit Anti-Complement fragment 3c Polyclonal Antibody (bs-6416R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Complement C3 Polyclonal Antibody, Unconjugated (bs-6416R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Complement C3 Polyclonal Antibody, Unconjugated (bs-6416R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.