bs-6370R-PerCP [Conjugated Primary Antibody]
p70 S6 kinase alpha Polyclonal Antibody, PerCP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: p70 S6 kinase alpha

Specificity: This antibody may detect p70 ribosomal S6 kinase beta 2.

Immunogen Range: 251-350/525


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6198

Swiss Prot: P23443

Source: KLH conjugated synthetic peptide derived from human p70 S6 kinase alpha

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B.

Conjugation: PerCP

Excitation/ Emission: 482nm/677nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 70


Cross Reactive Species: Human
Rat
Bovine
Others

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sung, Bokyung, et al. "Loquat leaf extract enhances myogenic differentiation, improves muscle function and attenuates muscle loss in aged rats."International Journal of Molecular Medicine.Read more>>
  • Li et al. Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling. (2014) Biosci.Rep. 34 Read more>>
  • Zhong et al. Camellia (Camellia oleifera Abel.) seed oil promotes milk fat and protein synthesis‐related gene expression in bovine mammary epithelial cells. Food Science & Nutrition. 2019.Read more>>
  • Yin H et al. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins (Basel). 2020 Jan 29;12(2). Read more>>
  • Li X et al. MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1. Biosci Rep. 2020 Jun 26;40(6):BSR20191327.Read more>>
  • Liu J. et al. Moxidectin induces Cytostatic Autophagic Cell Death of Glioma Cells through inhibiting the AKT/mTOR Signalling Pathway.. J Cancer. 2020 Aug;11(19):5802-5811Read more>>
  • Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1_ and _-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16Read more>>
  • Mao, Yushan. et al. Moxidectin induces autophagy arrest in colorectal cancer. MED ONCOL. 2022 Dec;39(12):1-11Read more>>
  • Gang Lin. et al. ARID1B blocks methionine-stimulated mTOR activation to inhibit milk fat and protein synthesis in and proliferation of mouse mammary epithelial cells. J NUTR BIOCHEM. 2023 Jan;:109274Read more>>
  • Wei Yu. et al. Glucose promotes cell growth and casein synthesis via ATF4/Nrf2-Sestrin2- AMPK-mTORC1 pathway in dairy cow mammary epithelial cells. ANIM BIOTECHNOL. 2023 Jul 12Read more>>
VALIDATION IMAGES