bs-6319R [Primary Antibody]
IL-1 Beta Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-1 Beta

Immunogen Range: 161-269/269


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3553

Swiss Prot: P01584

Source: KLH conjugated synthetic peptide derived from human IL-1 Beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 17/32


Cross Reactive Species: Human
Mouse
Rat
Bovine
Rabbit

Predicted Cross Reactive Species: Dog
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Dinesh, Palani, and MahaboobKhan Rasool. "Berberine, an isoquinoline alkaloid suppresses TXNIP mediated NLRP3 inflammasome activation in MSU crystal stimulated RAW 264.7 macrophages through the upregulation of Nrf2 transcription factor and alleviates MSU crystal induced inflammation in rats." International Immunopharmacology 44 (2017): 26-37.Read more>>
  • Han et al. Anti-inflammatory effect of polydeoxyribonucleotide on zoledronic acid-pretreated and lipopolysaccharide-stimulated RAW 264.7 cells. (2018) Exp.Ther.Med. 16:400-405Read more>>
  • Evashwick-Rogler et al. Inhibiting tumor necrosis factor-alpha at time of induced intervertebral disc injury limits long-term pain and degeneration in a rat model. (2018) JOR.Spine. 1 Read more>>
  • Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05.Read more>>
  • Yu H et al. Echinocystic acid, a natural plant extract, alleviates cerebral ischemia/reperfusion injury via inhibiting the JNK signaling pathway. Eur J Pharmacol. 2019 Oct 15;861:172610. Read more>>
  • Yang W et al. The Effect of Low and High Dose Deoxynivalenol on Intestinal Morphology, Distribution, and Expression of Inflammatory Cytokines of Weaning Rabbits. Toxins (Basel). 2019 Aug 13;11(8). pii: E473.Read more>>
  • Fukuda M et al. Disruption of P2X4 purinoceptor and suppression of the inflammation associated with cerebral aneurysm formation. J Neurosurg. 2019 Dec 20:1-13. Read more>>
  • Dai-Chao Maet al. Kv1.3 channel blockade alleviates cerebral ischemia/reperfusion injury by reshaping M1/M2 phenotypes and compromising the activation of NLRP3 inflammasome in microglia. Exp Neurol. 2020 Oct;332:113399.Read more>>
  • Zhang X et al. Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimers diseaseSci Adv.2020 Dec 4;6(49):eabb8680.Read more>>
  • Mengmeng Yang. et al. Mesenchymal stem cell-conditioned medium improved mitochondrial function and alleviated inflammation and apoptosis in non-alcoholic fatty liver disease by regulating SIRT1. Biochem Bioph Res Co. 2021 Mar;546:74Read more>>
  • Dai-Chao Ma. et al. Salvianolic Acids for Injection alleviates cerebral ischemia/reperfusion injury by switching M1/M2 phenotypes and inhibiting NLRP3 inflammasome/pyroptosis axis in microglia in vivo and in vitro. J Ethnopharmacol. 2021 Apr;270:113776Read more>>
  • Chinthalapally V. Rao. et al. GSK3\ARC/Arg3.1 and GSK3\Wnt signaling axes trigger amyloid\ accumulation and neuroinflammation in middle\aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221Read more>>
  • Wei\Cheng Chen. et al. Resistin enhances IL\1 and TNF\ expression in human osteoarthritis synovial fibroblasts by inhibiting miR\149 expression via the MEK and ERK pathways. Faseb J. 2020 Oct;34(10):13671-13684Read more>>
  • Mehmet Balbaba. et al. Anti-inflammatory effect of cortistatin in rat endotoxin-induced uveitis model. Indian J Ophthalmol. 2020 Sep; 68(9): 1920C1924Read more>>
  • Kun Hao. et al. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. Int J Biol Sci. 2020; 16(16): 3163C3173Read more>>
  • Jaclyn Iannucci. et al. Isoform-Specific Effects of Apolipoprotein E on Markers of Inflammation and Toxicity in Brain Glia and Neuronal Cells In Vitro. Curr Issues Mol Biol. 2021 Jun;43(1):215-225Read more>>
  • Abdelaziz Adam Idriss Arbab. et al. Metformin Inhibits Lipoteichoic AcidCInduced Oxidative Stress and Inflammation Through AMPK/NRF2/NF-B Signaling Pathway in Bovine Mammary Epithelial Cells. Front Vet Sci. 2021; 8: 66138Read more>>
  • Chenyu Zhang. et al. The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease. Redox Biol. 2021 Nov;47:102134Read more>>
  • Gul Fatma Yarim. et al. Nobiletin attenuates inflammation via modulating proinflammatory and antiinflammatory cytokine expressions in an autoimmune encephalomyelitis mouse model. Fitoterapia. 2022 Jan;156:105099Read more>>
  • Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-B signaling pathway. Neurochem Int. 2021 Nov;:105221Read more>>
  • Yu-Shen Luo. et al. Acrylonitrile exposure triggers ovarian inflammation and decreases oocyte quality probably via mitochondrial dysfunction induced apoptosis in mice. CHEM-BIOL INTERACT. 2022 Jun;360:109934Read more>>
  • Chao Huang. et al. Loss of TRIM67 Attenuates the Progress of Obesity-Induced Non-Alcoholic Fatty Liver Disease. INT J MOL SCI. 2022 Jan;23(13):7475Read more>>
  • Wu, Mengyu. et al. PTP1B Inhibitor Claramine Rescues Diabetes-Induced Spatial Learning and Memory Impairment in Mice. MOL NEUROBIOL. 2022 Nov;:1-21Read more>>
  • Ayano Nakai. et al. Development of a method to identify persistent and blanchable redness by skin blotting in mice. INT WOUND J. 2022 NovRead more>>
  • Zheng Xue. et al. Gastrodin improves neuroinflammation-induced cognitive dysfunction in rats by regulating NLRP3 inflammasome. BMC ANESTHESIOL. 2022 Dec;22(1):1-13Read more>>
  • Vittoria Borgonetti. et al. Zingiberene, a non-zinc-binding class I HDAC inhibitor: A novel strategy for the management of neuropathic pain. PHYTOMEDICINE. 2023 Mar;111:15467Read more>>
  • Yue Xiong. et al. Thymol improves autism-like behaviour in VPA-induced ASD rats through the Pin1/p38 MAPK pathway. INT IMMUNOPHARMACOL. 2023 Apr;117:109885Read more>>
  • Tian Jihua. et al. SIRT1 slows the progression of lupus nephritis by regulating the?NLRP3 inflammasome through ROS/TRPM2/Ca2+ channel. CLIN EXP MED. 2023 Jun;:1-14Read more>>
  • Shuhui Yu. et al. Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus. MOLECULES. 2023 Jan;28(12):4796Read more>>
  • Jihua Tian. et al. S1P/S1PR1 axis promotes macrophage M1 polarization through NLRP3 inflammasome activation in Lupus nephritis. MOL IMMUNOL. 2023 Aug;160:55Read more>>
  • Vittoria Borgonetti. et al. Honokiol-Rich Magnolia officinalis Bark Extract Attenuates Trauma-Induced Neuropathic Pain. ANTIOXIDANTS-BASEL. 2023 Aug;12(8):1518Read more>>
  • Xing Zeyu. et al. KPNA2 Silencing, Regulated by E3 Ubiquitin Ligase FBXW7, Alleviates Endothelial Dysfunction and Inflammation Through Inhibiting the Nuclear Translocation of p65 and IRF3: A Possible Therapeutic Approach for Atherosclerosis. INFLAMMATION. 2023 Jul;:1-18Read more>>
  • Fangda Fu. et al. AR/PCC herb pair inhibits osteoblast pyroptosis to alleviate diabetes-related osteoporosis by activating Nrf2/Keap1 pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 AugRead more>>
  • Yongbo Kang. et al. A Newly Synbiotic Combination Alleviates Obesity by Modulating the Gut MicrobiotaCFat Axis and Inhibiting the Hepatic TLR4/NF-B Signaling Pathway. MOL NUTR FOOD RES. 2023 Aug;:2300141Read more>>
  • Dilek ?evik. et al. Protective effect of pregabalin on renal and renal endothelial damage in sepsis induced by lipopolysaccharide. IMMUNOPHARM IMMUNOT. 2023 Sep 06Read more>>
  • Liangli Song. et al. Ginsenoside Rg1 Alleviates Lipopolysaccharide-Induced Fibrosis of Endometrial Epithelial Cells in Dairy Cows by Inhibiting Reactive Oxygen Species-Activated NLRP3. ANIMALS. 2023 Jan;13(23):3723Read more>>
  • Xianmou Fan. et al. A Multifunctional, Tough, Stretchable, and Transparent Curcumin Hydrogel with Potent Antimicrobial, Antioxidative, Anti-inflammatory, and Angiogenesis Capabilities for Diabetic Wound Healing. ACS APPL MATER INTER. 2024;16(8):9749C9767Read more>>
  • Mingwei Tong. et al. Serine protease inhibitor from the muscle larval Trichinella spiralis ameliorates non-alcoholic fatty liver disease in mice via anti-inflammatory properties and gut-liver crosstalk. BIOMED PHARMACOTHER. 2024 Mar;172:116223Read more>>
  • etl Seher Uysal. et al. Investigation of healing strategies in a rat corneal opacity model with polychromatic light and stem cells injection. J PHOTOCH PHOTOBIO B. 2024 Apr;253:112874Read more>>
  • Yue-Ming Zhang. et al. Mitochondrial antioxidant elamipretide improves learning and memory impairment induced by chronic sleep deprivation in mice. BRAIN BEHAV. 2024 Apr;14(5):e3508Read more>>
  • Zhou Xinghong. et al. Gut microbiota dysbiosis in hyperuricaemia promotes renal injury through the activation of NLRP3 inflammasome. MICROBIOME. 2024 Dec;12(1):1-2Read more>>
  • Nurten Alan. et al. Fig seed oil improves intestinal damage caused by 5-FU-induced mucositis in rats. FOOD SCI NUTR. 2024 JuRead more>>
  • Zeliu Huang. et al. Blocking 2-AR and Inhibiting COX-2: A Promising Approach to Suppress OSCC Development. INT DENT J. 2024 JulRead more>>
  • Amira Alashry Algammal. et al. Anticoccidial potentials of Azadirachta indica ethosomal nanovesicle in broiler chicks. VET PARASITOL. 2024 Oct;331:11027Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Lane 1: Mouse Raw264.7 Cells (30ug); Lane 2: Mouse Liver (40ug); Probed with IL-1 Beta Polyclonal Antibody, unconjugated (bs-6319R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Raw264.7 cell lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Recombinant mouse IL-1b protein, C-His probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.