bs-6313R-BF488 [Conjugated Primary Antibody]
4 Hydroxynonenal Polyclonal Antibody, AbBy Fluor® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: 4 Hydroxynonenal

Clonality: Polyclonal

Isotype: IgG

Source: KLH-conjugated 4-hydroxynonenal

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress 4-HNE has been hypothesized to play a key role in cell signal transduction, in a variety of pathways from cell cycle events to cellular adhesion.

Conjugation: AbBy Fluor® 488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 0.20229


Cross Reactive Species: Mouse
Rat
Others
Monkey
(4-Hydroxynonenal)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hong Duong, T. T., et al. "Pre‐Treatment With The Synthetic Antioxidant T‐Butyl Bisphenol Protects Cerebral Tissues From Experimental Ischemia Reperfusion Injury." Journal of Neurochemistry (2014).Read more>>
  • Haberman, Yael, et al. "Pediatric Crohn disease patients exhibit specific ileal transcriptome and microbiome signature." The Journal of Clinical Investigation 124.124 (8) (2014): 0-0.Read more>>
  • Zhang, Jing-Yao, et al. "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." World Journal of Gastroenterology 21.14 (2015): 4195-4209.Read more>>
  • Kagaya, Kenta, et al. "Involvement of oxidative stress in increased peripheral nerve firing during spontaneous dysesthesia in a mouse model of ischemia-reperfusion." Neuroscience Letters (2016).Read more>>
  • Sapkota, Arjun, et al. "Eupatilin exerts neuroprotective effects in mice with transient focal cerebral ischemia by reducing microglial activation." PloS one 12.2 (2017): e0171479.Read more>>
  • Polverino et al. A novel nonhuman primate model of cigarette smoke-induced airway disease. (2015) Am.J.Pathol. 185:741-55Read more>>
  • Vairappan B et al. Resveratrol Restores Neuronal Tight Junction Proteins Through Correction of Ammonia and Inflammation in CCl4-Induced Cirrhotic Mice. Molecular Neurobiology. Oct 30.Read more>>
  • Xiong J et al. (Pro) renin Receptor is Involved in Myocardial Damage in Alcoholic Cardiomyopathy. Alcohol Clin Exp Res. 2019 Sep 9.Read more>>
  • Sezer Z et al. Effects of vitamin E on nicotine-induced lipid peroxidation in rat granulosa cells: Folliculogenesis. Reprod Biol. 2020 Jan 6. pii: S1642-431X(19)30167-6. Read more>>
  • Zhang L et al. Neuroprotective effects of the novel GLP-1 long acting analogue semaglutide in the MPTP Parkinson's disease mouse model.Neuropeptides. 2018 Oct;71:70-80.Read more>>
  • Spinetti G et al. microRNA-21/PDCD4 Proapoptotic Signaling From Circulating CD34+ Cells to Vascular Endothelial Cells: A Potential Contributor to Adverse Cardiovascular Outcomes in Patients With Critical Limb Ischemia. Diabetes Care?2020 07;437(7)Read more>>
  • Chen-Yen Chienet al. Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats. Antioxidants (Basel). 2020 Jul 29;9(8):679.Read more>>
  • Chloe A Lowryet al. Expression of Neuronal Na+/K+-ATPase Subunit Isoforms in the MouseBrain Following Genetically Programmed or Behaviourally-induced Oxidative Stress. Neuroscience. 2020 Aug 21;442:202-215.Read more>>
  • Chou P et al. Sodium Thiosulfate Ameliorates Renovascular Hypertension-Induced Renal Dysfunction and Injury in RatsKidney Blood Press Res.2020 Dec 16;1-16.Read more>>
  • Honda Y et al. Augmentation of Bone Regeneration by Depletion of Stress-Induced Senescent Cells Using Catechin and Senolytics. Int J Mol Sci. 2020 Jun 13;21(12):4213.Read more>>
  • Kumar A et al. Nimbolide inhibits tumor growth by restoring hepatic tight junction protein expression and reduced inflammation in an experimental hepatocarcinogenesis. World J Gastroenterol.2020 Dec 7;26(45):7131-7152.Read more>>
  • Mehder Rasha H.. et al. Age-Related Neuronal Deterioration Specifically Within the Dorsal CA1 Region of the Hippocampus in a Mouse Model of Late Onset Alzheimers Disease. J Alzheimers Dis. 2021 Jan;79(4):1547-1561Read more>>
  • hakta Prasad Gaire. et al. BMS-986020, a Specific LPA1 Antagonist, Provides Neuroprotection against Ischemic Stroke in Mice. Antioxidants-Basel. 2020 Nov;9(11):1097Read more>>
  • Shotaro Hayashi. et al. Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice. Redox Biol. 2020 Oct;37:101726Read more>>
  • Sandro Sponga. et al. Preservation by cold storage vs ex vivo normothermic perfusion of marginal donor hearts: clinical, histopathologic, and ultrastructural features. J Heart Lung Transpl. 2020 Dec;39:1408Read more>>
  • Rashi Singhal. et al. HIF-2 activation potentiates oxidative cell death in colorectal cancers by increasing cellular iron. J Clin Invest. 2021 ApRead more>>
  • Hannah N. Bell. et al. Reuterin in the healthy gut microbiome suppresses colorectal cancer growth through altering redox balance. Cancer Cell. 2021 DecRead more>>
  • Cheng WJ. et al. <i>Astragalus mongholicus Bunge</i> Water Extract Exhibits Anti-inflammatory Effects in Human Neutrophils and Alleviates Imiquimod-Induced Psoriasis-Like Skin Inflammation in Mice.. Front Pharmacol. 2021 Dec;12:762829-762829Read more>>
  • Arjun Sapkota. et al. Oleanolic Acid Provides Neuroprotection against Ischemic Stroke through the Inhibition of Microglial Activation and NLRP3 Inflammasome Activation. Biomol Ther. 2022 Jan 1; 30(1): 55C63Read more>>
  • Hitoshi Maeda. et al. Nitric oxide facilitates the targeting Kupffer cells of a nano-antioxidant for the treatment of NASH. J Control Release. 2021 NovRead more>>
  • Xinchen Wang. et al. Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics. Int J Mol Sci. 2021 Jan;22(22):12415Read more>>
  • Min Hu. et al. Overactivation of the androgen receptor exacerbates gravid uterine ferroptosis via interaction with and suppression of the NRF2 defense signaling pathway. 2022 Jan 26Read more>>
  • Murakami, Mayuko. et al. Effectiveness of NLRP3 Inhibitor as a Non-Hormonal Treatment for ovarian endometriosis. Reprod Biol Endocrin. 2022 Dec;20(1):1-12Read more>>
  • Qiu Ming. et al. Interleukin 10 Attenuates Angiotensin II-Induced Aortic Remodelling by Inhibiting Oxidative Stress-Induced Activation of the Vascular p38 and NF-_B Pathways. OXID MED CELL LONGEV. 2022;2022:8244497Read more>>
  • Taisei Nagasaki. et al. A bioinspired carbon monoxide delivery system prevents acute kidney injury and the progression to chronic kidney disease. REDOX BIOL. 2022 Aug;54:102371Read more>>
  • Dong-Hyun Kim. et al. Farnesoid X receptor protects against cisplatin-induced acute kidney injury by regulating the transcription of ferroptosis-related genes. REDOX BIOL. 2022 Aug;54:102382Read more>>
  • Yang, Youjing. et al. STAT6 inhibits ferroptosis and alleviates acute lung injury via regulating P53/SLC7A11 pathway. CELL DEATH DIS. 2022 Jun;13(6):1-14Read more>>
  • Fangfang Huang. et al. STAT3-mediated ferroptosis is involved in ulcerative colitis. FREE RADICAL BIO MED. 2022 Aug;188:375Read more>>
  • Kai Xu. et al. GSK-J4, a Specific Histone Lysine Demethylase 6A Inhibitor, Ameliorates Lipotoxicity to Cardiomyocytes via Preserving H3K27 Methylation and Reducing Ferroptosis. FRONT CARDIOVASC MED. 2022; 9: 907747Read more>>
  • Di Zhang. et al. Colchicine improves severe acute pancreatitis-induced acute lung injury by suppressing inflammation, apoptosis and oxidative stress in rats. BIOMED PHARMACOTHER. 2022 Sep;153:113461Read more>>
  • Lopes, Natlia Medeiros Dias. et al. Role of papillary thyroid carcinoma patients with Hashimoto thyroiditis: evaluation of oxidative stress and inflammatory markers. CLIN TRANSL ONCOL. 2022 Jul;:1-13Read more>>
  • Yung-Fong Tsai. et al. Apremilast ameliorates acute respiratory distress syndrome by inhibiting neutrophil-induced oxidative stress. BIOMED J. 2022 SepRead more>>
  • Sam Cho. et al. Alpha-Lipoic Acid Attenuates Apoptosis and Ferroptosis in Cisplatin-Induced Ototoxicity via the Reduction of Intracellular Lipid Droplets. INT J MOL SCI. 2022 Jan;23(18):10981Read more>>
  • Weihuang Qiu. et al. Melatonin suppresses ferroptosis via activation of the Nrf2/HO-1 signaling pathway in the mouse model of sepsis-induced acute kidney injury. INT IMMUNOPHARMACOL. 2022 Nov;112:109162Read more>>
  • Eri Watanabe. et al. Drug library screening identifies histone deacetylase inhibition as a novel therapeutic strategy for choriocarcinoma. CANCER MED-US. 2022 SepRead more>>
  • Lianxiang Luo. et al. Astaxanthin attenuates ferroptosis via Keap1-Nrf2/HO-1 signaling pathways in LPS-induced acute lung injury. LIFE SCI. 2022 Oct;:121091Read more>>
  • Guiqin Bai. et al. Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress. EVID-BASED COMPL ALT. 2022 Oct 19;2022:58458Read more>>
  • o-Jeong Pyun. et al. Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells. ANTIOXIDANTS-BASEL. 2022 Nov;11(11):2256Read more>>
  • QianYu Lu. et al. Empagliflozin attenuates the renal tubular ferroptosis in diabetic kidney disease through AMPK/NRF2 pathway. FREE RADICAL BIO MED. 2023 Feb;195:89Read more>>
  • Huan Yang. et al. Elabela: Negative Regulation of Ferroptosis in Trophoblasts via the Ferritinophagy Pathway Implicated in the Pathogenesis of Preeclampsia. CELLS-BASEL. 2023 Jan;12(1):99Read more>>
  • Yung-Fong Tsai. et al. Carnosic acid inhibits reactive oxygen species-dependent neutrophil extracellular trap formation and ameliorates acute respiratory distress syndrome. LIFE SCI. 2022 Dec;:121334Read more>>
  • Lianxiang Luo. et al. ACSF2-mediated ferroptosis is involved in ulcerative colitis. LIFE SCI. 2022 Dec;:121272Read more>>
  • Nakata, Takahiro. et al. Effect of hormonal therapy on the otoconial changes caused by estrogen deficiency. SCI REP-UK. 2022 Dec;12(1):1-9Read more>>
  • Lianxiang Luo. et al. Identification of Lipocalin 2 as a Ferroptosis-Related Key Gene Associated with Hypoxic-Ischemic Brain Damage via STAT3/NF-&kappa;B Signaling Pathway. ANTIOXIDANTS-BASEL. 2023 Jan;12(1):186Read more>>
  • Ricarda Haustein. et al. C1q and Tumor Necrosis Factor Related Protein 9 Protects from Diabetic Cardiomyopathy by Alleviating Cardiac Insulin Resistance and Inflammation. CELLS-BASEL. 2023 Jan;12(3):443Read more>>
VALIDATION IMAGES