bs-6285R [Primary Antibody]
TMPRSS2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TMPRSS2

Immunogen Range: 301-400/492


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7113

Swiss Prot: O15393

Source: KLH conjugated synthetic peptide derived from human TMPRSS2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions; spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 54


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mohamed Elbadawy. et al. Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus. Int J Mol Sci. 2021 Jan;22(19):10763Read more>>
  • Samuel James Brake. et al. SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis. Int J Chronic Obstr. 2022 Jan;17:101-115Read more>>
  • Gi-Cheol Park. et al. ACE2 and TMPRSS2 Immunolocalization and COVID-19-Related Thyroid Disorder. BIOLOGY-BASEL. 2022 May;11(5):697Read more>>
  • Daichi Yamasoba. et al. Virological characteristics of the SARS-CoV-2 Omicron BA.2 spike. CELL. 2022 MayRead more>>
  • Tatiana F. R. Costa. et al. The ecotin-like peptidase inhibitor of Trypanosoma cruzi prevents TMPRSS2-PAR2-TLR4 crosstalk downmodulating infection and inflammation. FASEB J. 2024 Mar;38(6):e23566Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat colon labeled with Rabbit Anti-PRSS10 Polyclonal Antibody, Unconjugated (bs-6285R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Du145 cell lysates; Lane 2: Mouse Placenta lysates probed with PRSS10/TMPRSS2 Polyclonal Antibody, Unconjugated (bs-6285R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.