Host: Rabbit
Target Protein: CAMKK2
Immunogen Range: 501-588/588
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10645
Source: KLH conjugated synthetic peptide derived from human CAMKK2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
Pig
Sheep
Others
Predicted Cross Reactive Species:
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.Read more>>
- Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology 313 (2016): 149-158.Read more>>
- Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
- Yao C et al. Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs. Animals (Basel). 2019 Apr 1;9(4). pii: E137. Read more>>
- Yang WR et al. Oxidative stress mediates heat-induced changes of tight junction proteins in porcine Sertoli cells via inhibiting CaMKKβ-AMPK pathway. Theriogenology. 2019 Sep 19;142:104-113. Read more>>
- Zhang Q et al. CTRP13 attenuates the expression of LN and CAV-1 Induced by high glucose via CaMKK/AMPK pathway in rLSECs. Aging (Albany NY). 2020 Jun 17;12(12):11485-11499.Read more>>
- Zuodong Chen. et al. Oxidative stress induced by hydrogen peroxide promotes glycolysis by activating CaMKK/LKB1/AMPK pathway in broiler breast muscle. Poultry Sci. 2021 Dec;:101681Read more>>
- Yuge Jiang. et al. Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake.. FRONT NUTR. 2022 Nov;9:1039753-1039753Read more>>
- Xing Guo. et al. Microcystin leucine arginine induces human sperm damage: Involvement of the Ca2+/CaMKK/AMPK pathway. ECOTOX ENVIRON SAFE. 2023 May;256:114845Read more>>
Lane 1: Hela cell lysates probed with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebral cortex cell lysates; Lane 2: Rat Cerebral cortex cell lysates probed with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CAMKK2 Polyclonal Antibody(bs-6253R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Human Jurkat cell lysates; Lane 2: Human MOLT4 cell lysates; Lane 3: Human HepG2 cell lysates probed with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.