Host: Rabbit
Target Protein: Wnt8b
Immunogen Range: 131-230/351
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7479
Swiss Prot: Q93098
Source: KLH conjugated synthetic peptide derived from human Wnt8b
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 36
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Pig
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1:A549 cell lysates probed with Rabbit Anti-Wnt8b Polyclonal Antibody, Unconjugated (bs-6245R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Wnt8b Polyclonal Antibody, Unconjugated (bs-6245R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Wnt8b Polyclonal Antibody, Unconjugated (bs-6245R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse ovary lysates probed with Wnt8b Polyclonal Antibody, Unconjugated (bs-6245R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.