VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with NARC1 Polyclonal Antibody (bs-6060R) at 1:400 overnight at 4°C, followed by a SP kit and DAB staining.
Lane 1: Human A431 cell lysates; Lane 2: Mouse Uterus lysates probed with NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human SW480 cell lysates probed with NARC1 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1) Polyclonal Antibody, Unconjugated (bs-6060R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Recombinant human PCSK9 protein, C-His (HEK293)( bs-43105P) probed with NARC1/PCSK9 Polyclonal Antibody, Unconjugated (bs-6060R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.