bs-6057R [Primary Antibody]
METTL11A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: METTL11A

Immunogen Range: 25-130/223


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 28989

Swiss Prot: Q9BV86

Source: KLH conjugated synthetic peptide derived from human METTL11A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 25


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti-METTL11A Polyclonal Antibody, Unconjugated (bs-6057R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with METTL11A Polyclonal Antibody (bs-6057R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with METTL11A Polyclonal Antibody (bs-6057R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with METTL11A Polyclonal Antibody, Unconjugated (bs-6057R) at 1:200 overnight at 4°C, DAB staining.