bs-60150R [Primary Antibody]
Mono-Methyl-Histone H2B (Lys5) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Mono-Methyl-Histone H2B (Lys5)

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P62807

Purification: Protein A coupled with the antigen peptide containing H2BK5me1.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:300-5000)

Predicted Molecular Weight: 14


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: H1299, MCF-7, C2C12, rH2B Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa