Host: Rabbit
Target Protein: Histone H3 (tri methyl K4, serotonyl Q5)
Clonality: Polyclonal
Isotype: IgG
Swiss Prot: P68431
Purification: Protein A coupled with the antigen peptide containing H3K4me3/Q5ser.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: WB(1:300-5000)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: lane 1: PTM-1420+H3K4me3/Q5ser peptides, lane 2: PTM-1420+unmodified peptides, lane 3: PTM-1420, 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: N2a Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa