Host: Rabbit
Target Protein: Histone H3 (tri methyl K9, phospho S10)
Clonality: Polyclonal
Isotype: IgG
Swiss Prot: P68431
Purification: Protein A coupled with the antigen peptide containing H3K9me3/S10ph.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-) JK, (+) JK+Etopside (100ug/ml, 24hr) +Calyculin A (100nM, 1hr) Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 17 kDa Observed MW: 17 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-) C2C12, (+) C2C12+Nocodazole (100ng/ml, 18hr) +Calyculin A (100nM, 1hr) Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 17 kDa Observed MW: 17 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Mouse spleen, Mouse liver Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 17 kDa Observed MW: 17 kDa
Tissue: Human neuroblastoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bs-60074R