bs-5921R-FITC [Conjugated Primary Antibody]
YBX-1 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: YBX-1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4904

Source: KLH conjugated synthetic peptide derived from human YBX-1/YB1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumour aggressiveness and belongs to the cold-shock domain family.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse kidney cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with YBX-1 Polyclonal Antibody(bs-5921R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).