bs-5921R [Primary Antibody]
YBX-1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: YBX-1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4904

Source: KLH conjugated synthetic peptide derived from human YBX-1/YB1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumour aggressiveness and belongs to the cold-shock domain family.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti YBX-1/YB1 Polyclonal Antibody, Unconjugated (bs-5921R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human brain glioblastoma labeled with Rabbit Anti YBX-1/YB1 Polyclonal Antibody, Unconjugated (bs-5921R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with YBX-1 Polyclonal Antibody, Unconjugated (bs-5921R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with YBX-1 Polyclonal Antibody, Unconjugated (bs-5921R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Human U251 cell lysates probed with YB1 Polyclonal Antibody, Unconjugated (bs-5921R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.