bs-5913R
[Primary Antibody]
Calreticulin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Calreticulin
Immunogen Range: 101-200/417
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 811
Swiss Prot: P27797
Source: KLH conjugated synthetic peptide derived from human Calreticulin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 44
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Chicken
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Angelova, Assia L., et al. "Complementary induction of immunogenic cell death by oncolytic parvovirus H-1PV and gemcitabine in pancreatic cancer." Journal of Virology (2014): JVI-03688.Read more>>
- Chen, Beilei, et al. "Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-reperfusion Injury." BioMed Research International.Read more>>
- Tanaka, Mamoru, et al. "Immunogenic cell death due to a new photodynamic therapy (PDT) with glycoconjugated chlorin (G-chlorin)." Oncotarget (2016).Read more>>
- Erbas et al. Oxytocin alleviates cisplatin-induced renal damage in rats. (2014) Iran.J.Basic.Med.Sci. 17:747-52Read more>>
- An J et al. Nanoenabled Disruption of Multiple Barriers in Antigen Cross-Presentation of Dendritic Cells via Calcium Interference for Enhanced Chemo-Immunotherapy. ACS Nano 2020 06 23;146(6)Read more>>
- Rui X et al. Imperative and effective reversion of synovial hyperplasia and cartilage destruction in rheumatoid arthritis through multiple synergistic effects of O2 and Ca2+. Mater Sci Eng C Mater Biol Appl.2020 Sep;114:111058.Read more>>
- Hiroshi Fukushima. et al. Combination of Cisplatin and Irradiation Induces Immunogenic Cell Death and Potentiates Postirradiation AntiCPD-1 Treatment Efficacy in Urothelial Carcinoma. Int J Mol Sci. 2021 Jan;22(2):535Read more>>
- Hiroshi Fukushima. et al. Combination of Cisplatin and Irradiation Induces Immunogenic Cell Death and Potentiates Postirradiation AntiCPD-1 Treatment Efficacy in Urothelial Carcinoma. Int J Mol Sci. 2021 Jan;22(2):535Read more>>
- Qilin Li. et al. IDO-inhibitor potentiated immunogenic chemotherapy abolishes primary tumor growth and eradicates metastatic lesions by targeting distinct compartments within tumor microenvironment. Biomaterials. 2021 Feb;269:120388Read more>>
- Kai Sun. et al. Reinforcing the Induction of Immunogenic Cell Death Via Artificial Engineered Cascade Bioreactor-Enhanced Chemo-Immunotherapy for Optimizing Cancer Immunotherapy. 2021 Aug 06Read more>>
- Chavez-Dominguez RL. et al. Role of HMGB1 in Cisplatin-Persistent Lung Adenocarcinoma Cell Lines.. Front Oncol. 2021 Dec;11:750677-750677Read more>>
- Yuting Shen. et al. Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation. Acs Nano. 2021;XXXX(XXX):XXX-XXXRead more>>
- Ruikun Wang. et al. CD40L-armed oncolytic herpes simplex virus suppresses pancreatic ductal adenocarcinoma by facilitating the tumor microenvironment favorable to cytotoxic T cell response in the syngeneic mouse model. J Immunother Cancer. 2022 Jan;10(1):e003809Read more>>
- owei Chen. et al. iRGD Tumor-Penetrating Peptide-Modified Nano-Delivery System Based on a Marine Sulfated Polysaccharide for Enhanced Anti-Tumor Efficiency Against Breast Cancer. Int J Nanomed. 2022 Feb;17:617-633Read more>>
- Zhenzhen Chen. et al. Photothermal MnO2 nanoparticles boost chemo-photothermal therapy-induced immunogenic cell death in tumor immunotherapy. Int J Pharmaceut. 2022 Apr;617:121578Read more>>
- Xiu Zhao. et al. Intracellular Self-Assembly Driven Nucleus-Targeted Photo-Immune Stimulator with Chromatin Decompaction Function for Robust Innate and Adaptive Antitumor Immunity. Adv Funct Mater. 2022 Apr;32(17):2108883Read more>>
- Wei Jiang. et al. Nitric Oxide Induces Immunogenic Cell Death and Potentiates Cancer Immunotherapy. Acs Nano. 2022;XXXX(XXX):XXX-XXXRead more>>
- Feng Liu. et al. LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy. Biomaterials. 2022 May;284:121485Read more>>
- Pan Zhang. et al. The programmed site-specific delivery of LY3200882 and PD-L1 siRNA boosts immunotherapy for triple-negative breast cancer by remodeling tumor microenvironment. BIOMATERIALS. Biomaterials. 2022 Apr;:121518Read more>>
- Kai Sun. et al. Salicylic acid-based hypoxia-responsive chemodynamic nanomedicines boost antitumor immunotherapy by modulating immunosuppressive tumor microenvironment. ACTA BIOMATER. 2022 JuRead more>>
- Shohei Katsuki. et al. Radiation therapy enhances systemic antitumor efficacy in PD-L1 therapy regardless of sequence of radiation in murine osteosarcoma. PLOS ONE. 2022 Jul;17(7):e0271205Read more>>
- Nan Zhang. et al. Co-Delivery of Doxorubicin and Anti-PD-L1 Peptide in Lipid/PLGA Nanocomplexes for the Chemo-Immunotherapy of Cancer. MOL PHARMACEUT. 2022;19(9):34393449Read more>>
- Yanhua Zhai. et al. Self-activated arsenic manganite nanohybrids for visible and synergistic thermo/immuno-arsenotherapy. J CONTROL RELEASE. 2022 Oct;350:761Read more>>
- Axel Berg-Larsen. et al. Tumor growth inhibition and immune system activation following treatment with thorium-227 conjugates and PD-1 check-point inhibition in the MC-38 murine model.. FRONT MED-LAUSANNE. 2022 Nov;9:1033303-1033303Read more>>
- Nguyen Thi Nguyen. et al. Amplified Fenton-Based Oxidative Stress Utilizing Ultraviolet Upconversion Luminescence-Fueled Nanoreactors for Apoptosis-Strengthened Ferroptosis Anticancer Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXXRead more>>
- Qilin Li. et al. NIR-responsive hollow germanium nanospheres mediate photothermal/photodynamic therapy and restrain immunosuppression to cooperatively eradicate primary and metastatic tumors. CHEM ENG J. 2023 Jan;:141314Read more>>
- Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682Read more>>
- Ting-Ting Yu. et al. Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage. INT IMMUNOPHARMACOL. 2023 Feb;115:109661Read more>>
- Xiao Wu. et al. Nanodrug constructed using dietary antioxidants for immunotherapy of metastatic tumors. J MATER CHEM B. 2023 FebRead more>>
- Xianquan Feng. et al. Drug Self-Delivery Nanocubes Enhance O2-Economized Photodynamic-Immunotherapy of Triple-Negative Breast Cancer by Downregulating Wnt/-catenin Signaling. ADV HEALTHC MATER. 2023 Apr;:2203019Read more>>
- Kai Sun. et al. Photothermal enhanced polyphenol-based nanofibers ameliorate catalytic efficiency of ferroptosis for synergistic tumor therapy. CHEM ENG J. 2023 Aug;470:14436Read more>>
- Shengchang Tao. et al. Dendrobium officinale polysaccharide-based carrier to enhance photodynamic immunotherapy. CARBOHYD POLYM. 2023 Oct;317:121089Read more>>
- Ziyang Zhu. et al. Aggregation-Induced Emission Photosensitizer/Bacteria Biohybrids Enhance Cerenkov Radiation-Induced Photodynamic Therapy by Activating Anti-Tumor Immunity for Synergistic Tumor Treatment. ACTA BIOMATER. 2023 JuRead more>>
- Shiyu Liu. et al. OX40L-Armed Oncolytic Virus Boosts T-cell Response and Remodels Tumor Microenvironment for Pancreatic Cancer Treatment. THERANOSTICS. 2023; 13(12): 4016C4029Read more>>
- Fukushima Hiroshi. et al. Intratumoral interleukin-15 improves efficacy of near-infrared photoimmunotherapy. MOL CANCER THER. 2023 JulRead more>>
- Liu-Gen Li. et al. A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage. ADV HEALTHC MATER. 2023 Aug;:2301561Read more>>
- Ziwen Qiu. et al. Self-reinforced photodynamic immunostimulator to downregulate and block PD-L1 for metastatic breast cancer treatment. BIOMATERIALS. 2023 Dec;303:122392Read more>>
- Peng Xue. et al. Fe3+ mediated shikonin and PPA coloaded liposomes induce robust immunogenic cell death by integrating ROS enhancement and GSH depletion. INT J PHARMACEUT. 2024 Jan;649:123657Read more>>
- Yaoqi Li. et al. Long-Acting Nanohybrid Hydrogel Induces Persistent Immunogenic Chemotherapy for Suppressing Postoperative Tumor Recurrence and Metastasis. MOL PHARMACEUT. 2023;XXXX(XXX):XXX-XXXRead more>>
- Wangxian Fu. et al. A programmable releasing versatile hydrogel platform boosts systemic immune responses via sculpting tumor immunogenicity and reversing tolerogenic dendritic cells. BIOMATERIALS. 2024 Mar;305:122444Read more>>
- Ren-Jiang Kong. et al. Self-Delivery Photodynamic Re-educator Enhanced Tumor Treatment by Inducing Immunogenic Cell Death and Improving Immunosuppressive Microenvironments. ACS APPL MATER INTER. 2023;15(51):59165C59174Read more>>
- Zi-Wen Qiu. et al. Breaking Physical Barrier of Fibrotic Breast Cancer for Photodynamic Immunotherapy by Remodeling Tumor Extracellular Matrix and Reprogramming Cancer-Associated Fibroblasts. ACS NANO. 2024;18(13):9713C9735Read more>>
- Yunkun Li. et al. Dendritic nanomedicine enhances chemo-immunotherapy by disturbing metabolism of cancer-associated fibroblasts for deep penetration and activating function of immune cells. ACTA PHARM SIN B. 2024 MaRead more>>
- Yang Huizhen. et al. Self-delivery photothermal-boosted-nanobike multi-overcoming immune escape by photothermal/chemical/immune synergistic therapy against HCC. J NANOBIOTECHNOL. 2024 Dec;22(1):1-18Read more>>
- Yingcai Meng. et al. Mn-phenolic networks as synergistic carrier for STING agonists in tumor immunotherapy. MATER TODAY BIO. 2024 Jun;26:101018Read more>>
- Liu-Gen Li. et al. Dihydroartemisinin-driven TOM70 inhibition leads to mitochondrial destabilization to induce pyroptosis against lung cancer. PHYTOTHER RES. 2024 MayRead more>>
- Sha Xu. et al. Approaches to selective and potent inhibition of glioblastoma by vanadyl complexes: Inducing mitotic catastrophe and methuosis. J INORG BIOCHEM. 2024 Aug;257:11261Read more>>
- Hongxin Liu. et al. Tumor associated antigens combined with carbon dots for inducing durable antitumor immunity. J COLLOID INTERF SCI. 2024 Nov;673:594Read more>>
- Jingru Wang. et al. Zinc oxide nanoparticles with catalase-like nanozyme activity and near-infrared light response: A combination of effective photodynamic therapy, autophagy, ferroptosis, and antitumor immunity. ACTA PHARM SIN B. 2024 JulRead more>>
- Haijiao Wang. et al. Catalase-positive Staphylococcus epidermidis based cryo-millineedle platform facilitates the photo-immunotherapy against colorectal cancer via hypoxia improvement. J COLLOID INTERF SCI. 2024 Dec;676:506Read more>>
VALIDATION IMAGES
HeLa cells probed with Rabbit Anti-Calreticulin Polyclonal Antibody (bs-5913R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.
Lane 1: Mouse Liver cell lysates probed with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.01%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Calreticulin Polyclonal Antibody(bs-5913R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human SH-SY5Y cell lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human HL-60 cell lysates; Lane 8: Human A431 cell lysates probed with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.