DATASHEET
Host:
Rabbit
Target Protein:
CYPA
Immunogen Range:
101-165/165
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5478
Swiss Prot:
P62937
Source:
KLH conjugated synthetic peptide derived from human Cyclophilin A.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
PRODUCT SPECIFIC PUBLICATIONS
- Kang, Taewook, et al. "Large-scale analysis of posttranslational modifications in the hippocampus of patients with Alzheimer’s disease using pI shift and label-free quantification without enrichment." Analytical and Bioanalytical Chemistry (2014): 1-14.Read more>>
- Yang, Chenying, et al. "MULTISPECTRAL WIDE-FIELD ENDOSCOPIC IMAGING OF FLUORESCENCE." U.S. Patent No. 20,150,216,398. 6 Aug. 2015.Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-CYPA Polyclonal Antibody, Unconjugated (bs-5912R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Mouse NIH/3T3 cell lysates; Lane 5: Rat Thymus tissue lysates; Lane 6: Rat Lymph node tissue lysates; Lane 7: Rat Lung tissue lysates; Lane 8: Human Hela cell lysates; Lane 9: Human PANC-1 cell lysates; Lane 10: Human Molt-4 cell lysates; Lane 11: Human HUVEC cell lysates; Lane 12: Human THP-1 cell lysates probed with CYPA Polyclonal Antibody, Unconjugated (bs-5912R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.