Host: Rabbit
Target Protein: GADD45B
Immunogen Range: 81-160/160
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4616
Swiss Prot: O75293
Source: KLH conjugated synthetic peptide derived from human GADD45B.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 18
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Horse
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Haditsch, Ursula, et al. "Neuronal Rac1 Is Required for Learning-Evoked Neurogenesis." The Journal of Neuroscience 33.30 (2013): 12229-12241.Read more>>
- Ming Xie. et al. GADD45A and GADD45B as Novel Biomarkers Associated with Chromatin Regulators in Renal Ischemia-Reperfusion Injury. INT J MOL SCI. 2023 Jan;24(14):11304Read more>>
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-GADD45B Polyclonal Antibody, Unconjugated (bs-5904R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-GADD45B Polyclonal Antibodyy, Unconjugated (bs-5904R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GADD45B Polyclonal Antibody (bs-5904R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GADD45B Polyclonal Antibody(bs-5904R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Liver tissue lysates; Lane 3: Rat Kidney tissue lysates probed with GADD45B Polyclonal Antibody, Unconjugated (bs-5904R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.