DATASHEET
Host:
Rabbit
Target Protein:
ADORA2B
Immunogen Range:
101-200/332
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
136
Swiss Prot:
P29275
Source:
KLH conjugated synthetic peptide derived from human ADORA2B
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Conjugation:
FITC
Excitation/ Emission:
494nm/518nm
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
37
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Pig
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Shi L et al. Adenosine Interaction with Adenosine Receptor A2a Promotes Gastric Cancer Metastasis by Enhancing PI3K–AKT–mTOR Signaling. Mol Biol Cell. 2019 Jul 24:mbcE19030136. Read more>>
- Alina V. Meyer. et al. Host CD39 Deficiency Affects Radiation-Induced Tumor Growth Delay and Aggravates Radiation-Induced Normal Tissue Toxicity. Front Oncol. 2020; 10: 554883Read more>>
- Monserrat Carrera-Martnez. et al. Inhibition of CD73 expression or A2AR blockade reduces MRP1 expression and increases the sensitivity of cervical cancer cells to cisplatin. CELL BIOCHEM FUNCT. 2023 FebRead more>>
- Gaona Shi. et al. Bone-derived MSCs encapsulated in alginate hydrogel prevent collagen-induced arthritis in mice through the activation of adenosine A2A/2B receptors in tolerogenic dendritic cells. ACTA PHARM SIN B. 2023 ApRead more>>
- Xue-qi Liu. et al. Blocking ATP-P1Rs axis attenuate alcohol-related liver fibrosis. LIFE SCI. 2023 Jun;:121896Read more>>
- ing Wang. et al. Co-inhibition of adenosine 2b receptor and programmed death-ligand 1 promotes the recruitment and cytotoxicity of natural killer cells in oral squamous cell carcinoma. PEERJ. 2023 Aug;11:e15922Read more>>
VALIDATION IMAGES
A431 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADORA2B Polyclonal Antibody(bs-5900R-FITC)at 1:50dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).