Host: Rabbit
Target Protein: ADORA2B
Immunogen Range: 101-200/332
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 136
Swiss Prot: P29275
Source: KLH conjugated synthetic peptide derived from human ADORA2B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 37
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Pig
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Shi L et al. Adenosine Interaction with Adenosine Receptor A2a Promotes Gastric Cancer Metastasis by Enhancing PI3K–AKT–mTOR Signaling. Mol Biol Cell. 2019 Jul 24:mbcE19030136. Read more>>
- Alina V. Meyer. et al. Host CD39 Deficiency Affects Radiation-Induced Tumor Growth Delay and Aggravates Radiation-Induced Normal Tissue Toxicity. Front Oncol. 2020; 10: 554883Read more>>
- Monserrat Carrera-Martnez. et al. Inhibition of CD73 expression or A2AR blockade reduces MRP1 expression and increases the sensitivity of cervical cancer cells to cisplatin. CELL BIOCHEM FUNCT. 2023 FebRead more>>
- Gaona Shi. et al. Bone-derived MSCs encapsulated in alginate hydrogel prevent collagen-induced arthritis in mice through the activation of adenosine A2A/2B receptors in tolerogenic dendritic cells. ACTA PHARM SIN B. 2023 ApRead more>>
- Xue-qi Liu. et al. Blocking ATP-P1Rs axis attenuate alcohol-related liver fibrosis. LIFE SCI. 2023 Jun;:121896Read more>>
- ing Wang. et al. Co-inhibition of adenosine 2b receptor and programmed death-ligand 1 promotes the recruitment and cytotoxicity of natural killer cells in oral squamous cell carcinoma. PEERJ. 2023 Aug;11:e15922Read more>>
RSC96 cells probed with Rabbit Anti-ADORA2B Polyclonal Antibody, PE Conjugated (bs-5900R-PE) at 1:30 for 40 minutes (green) compared to control cells (blue).
Mouse brain lysates probed with Rabbit Anti-ADORA2B Polyclonal Antibody (bs-5900R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4°C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with ADORA2B polyclonal antibody, unconjugated (bs-5900R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.