bs-5890R [Primary Antibody]
Lumican Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Lumican

Immunogen Range: 61-160/338


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4060

Swiss Prot: P51884

Source: KLH conjugated synthetic peptide derived from human Lumican

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 35


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yam, GH., et al. “Ex Vivo Propagation of Human Corneal Stromal “Activated Keratocytes” for Tissue Engineering.” Cell Transplantation (2015); 24(9): 1845-1861.Read more>>
  • inbin He. et al. 3D printed biomimetic epithelium/stroma bilayer hydrogel implant for corneal regeneration. Bioact Mater. 2022 JaRead more>>
  • Ruixing Liu. et al. Celastrol Alleviates Corneal Stromal Fibrosis by Inhibiting TGF-1/Smad2/3-YAP/TAZ Signaling After Descemet Stripping Endothelial Keratoplasty. INVEST OPHTH VIS SCI. 2023 Mar;64(3):9-9Read more>>
  • Shuo Jia. et al. Digital light processing-bioprinted poly-NAGA-GelMA-based hydrogel lenticule for precise refractive errors correction. BIOFABRICATION. 2023 ApRead more>>
  • Riau Andri K.. et al. Impact of keratocyte differentiation on corneal opacity resolution and visual function recovery in male rats. NAT COMMUN. 2024 Jun;15(1):1-17Read more>>
VALIDATION IMAGES

Antigen: bs-5890P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450