bs-5856R [Primary Antibody]
ADAMTS13 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: ADAMTS13

Immunogen Range: 401-500/1427


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11093

Swiss Prot: Q76LX8

Source: KLH conjugated synthetic peptide derived from human ADAMTS13

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 145


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Masakazu Saeki. et al. Assaying ADAMTS13 Activity as a Potential Prognostic Biomarker for Sinusoidal Obstruction Syndrome in Mice. INT J MOL SCI. 2023 Jan;24(22):16328Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-ADAMTS13 Polyclonal Antibody, Unconjugated (bs-5856R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Liver; 40ug loaded into the lane; Probed with ADAMTS13 Polyclonal Antibody, unconjugated (bs-5856R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.