Host: Rabbit
Target Protein: ADAM12
Immunogen Range: 201-300/909
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8038
Source: KLH conjugated synthetic peptide derived from human ADAM12
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Predicted Molecular Weight: 77/100
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Miranda da Costa NM et al. HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways. Cells. 2019 Jul 17;8(7). pii: E731.Read more>>
Mouse heart lysates probed with ADAM12 Polyclonal Antibody, Unconjugated (bs-5847R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U-87MG cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM12 Antibody（bs-5847R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).