bs-5810R [Primary Antibody]
Lactoferrin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Lactoferrin

Immunogen Range: 611-710/710


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4057

Source: KLH conjugated synthetic peptide derived from human Lactoferrin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 76


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao et al. Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhage. (2017) Nat.Commun. 8:602Read more>>
  • Carman Man et al. Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA SequencingCell Rep.2020 Dec 29;33(13):108566.Read more>>
  • Tsatsanis, Andrew. et al. The acute phase protein lactoferrin is a key feature of Alzheimers disease and predictor of A burden through induction of APP amyloidogenic processing. Mol Psychiatr. 2021 Aug;:1-16Read more>>
  • Zhongnan Xiao. et al. Reduction of lactoferrin aggravates neuronal ferroptosis after intracerebral hemorrhagic stroke in hyperglycemic mice. Redox Biol. 2022 Apr;50:102256Read more>>
VALIDATION IMAGES

Human breast milk probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n


Human Lactoferrin protein (100ng) lysates probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Polyclonal Antibody(bs-5810R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).