bs-5805R [Primary Antibody]
RIPK-1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RIPK-1

Immunogen Range: 581-671/671


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8737

Swiss Prot: Q13546

Source: KLH conjugated synthetic peptide derived from human RIPK-1/RIP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 74


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Luo, Yuhao, et al. "Lycorine induces programmed necrosis in the multiple myeloma cell line ARH-77." Tumor Biology (2014): 1-9.Read more>>
  • Seifert, Lena, et al. "The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression." Nature (2016).Read more>>
  • Yang B et al. Polychlorinated Biphenyl Quinone Promotes Macrophage-Derived Foam Cell Formation. Chem Res Toxicol. 2019 Nov 13.Read more>>
  • Qin Yang. et al. Gypenoside XLIX protects against acute kidney injury by suppressing IGFBP7/IGF1R-mediated programmed cell death and inflammation. Phytomedicine. 2021 Mar;:153541Read more>>
  • Tiziano A Schweizer. et al. Blunted sFasL signalling exacerbates TNF-driven neutrophil necroptosis in critically ill COVID-19 patients. Clin Transl Immunol. 2021 Jan;10(12):e1357Read more>>
  • Lei Lei. et al. Selenium Deficiency-Induced Oxidative Stress Causes Myocardial Injury in Calves by Activating Inflammation, Apoptosis, and Necroptosis. ANTIOXIDANTS-BASEL. 2023 Feb;12(2):229Read more>>
  • Shuang Wang. et al. Paricalcitol Ameliorates Acute Kidney Injury in Mice by Suppressing Oxidative Stress and Inflammation via Nrf2/HO-1 Signaling. INT J MOL SCI. 2023 Jan;24(2):969Read more>>
  • Serdar Bat?kan Kavukcu. et al. Cell Death Mechanism of Organometallic Ruthenium(II) and Iridium(III) Arene Complexes on HepG2 and Vero Cells. ACS OMEGA. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Faika Ba?o?lu-nal. et al. A newly synthesized thiosemicarbazide derivative trigger apoptosis rather than necroptosis on HEPG2 cell line. CHEM BIOL DRUG DES. 2023 SepRead more>>
  • Shasha Chen. et al. Quercetin alleviates zearalenone-induced apoptosis and necroptosis of porcine renal epithelial cells by inhibiting CaSR/CaMKII signaling pathway. FOOD CHEM TOXICOL. 2023 Nov;:114184Read more>>
  • Tohru Mutoh. et al. Spatiotemporal expression patterns of ZBP1 in the brain of mouse experimental stroke model. J CHEM NEUROANAT. 2023 Dec;134:102362Read more>>
  • Huang Meimei. et al. METTL3 restricts RIPK1-dependent cell death via the ATF3-cFLIP axis in the intestinal epithelium. Cell Regeneration. 2024 Dec;13(1):1-15Read more>>
VALIDATION IMAGES

Lane 1: Raji cell lysates probed with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum cell lysates probed with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (RIPK-1 ) Polyclonal Antibody, Unconjugated (bs-5805R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.