bs-5786R [Primary Antibody]
KLST Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KLST

Immunogen Range: 151-250/427


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5267

Source: KLH conjugated synthetic peptide derived from human KLST/Kallistatin (161-220aa)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Kallistatin is a serine proteinase inhibitor which binds to tissue kallikrein and inhibits its amidolytic and kininogenase activity. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 49


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Biswas et al. Polymorphisms at the F12 and KLKB1 loci have significant trait association with activation of the renin-angiotensin system. (2016) BMC.Med.Genet. 17:21Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with KLST Polyclonal Antibody, Unconjugated (bs-5786R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Pancreas lysates; Lane 2: Mouse Kidney lysates; Lane 3: Mouse Lymph node lysates probed with KLST Polyclonal Antibody, Unconjugated (bs-5786R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.